改良CTAB-LiCl法提取枣总RNA体系的建立  

Establishment of Improved CTAB-LiCl Optimum System on Isolating Total RNA of Chinese Jujube(Ziziphus jujuba Mill.)

作　　者：宋蓓[1] 赵锦[1] 刘孟军[2] 薛渝峰[2] 

机构地区：[1]河北农业大学生命科学学院,河北保定071001 [2]河北农业大学中国枣研究中心,河北保定071001

出　　处：《中国农学通报》2007年第7期79-83,共5页Chinese Agricultural Science Bulletin

基　　金：国家科技攻关项目"枣树优良新品种选育与发展战略研究"(No.2001BA502B09-04);河北省自然科学基金"抗枣疯病相关基因DDRT-PCR研究"(No.c2005000267);河北农业大学科技将帅计划;河北农业大学科学发展基金资助

摘　　要：试验以枣树田间枝条的枝皮及组培苗为材料,应用改进的CTAB法进行了总RNA的提取。针对枣组织中富含多糖多酚的特点,改进了CTAB法,采用β-巯基乙醇和PVP去除酚类,KAc及无水乙醇去除多糖,LiCl沉淀过夜的方法提取得到的RNA,条带整齐、清晰,A260/A280介于1.8～2.0之间,A260/A230均大于2.0,证明得到的RNA纯度、完整性和产率均较高,蛋白、多糖及酚类等去除较彻底。结果表明,本试验建立的改良CTAB-LiCl法适于进行枣总RNA的提取。In this paper, the branches of Chinese jujube and its plantlets were used as test materials to establish the optimum system of isolating total RNA. Because Chinese jujube are rich in polysaccharides and polyphenol compounds, the CTAB method were improved by added polyvinylpyrrolidone（PVP） and β- ME to remove polyphenol compounds, added KAc and ethanol to remove polysaccharides and added LiCl to deposit RNA. The bands of the isolated RNA were regular and clear. The A260/A280 of total RNA were between 1.8-2.0 and A260/A230 were more than 2.0. That proved the method could extract highpurity and highly-complete RNA at a high productivity and could remove protein, polysaccharides and polyphenol compounds completely. The result showed that the improved CTAB-LiCl method is suitable to isolate total RNA of Chinese jujube.

关 键 词：枣 总RNA提取 CTAB法 LiCl沉淀 

分 类 号：Q522[生物学—生物化学] Q503