HBV S基因修饰树突状细胞疫苗诱导特异性CTL反应的探讨  

Induction of specific cytotoxic T lymphocyte response against hepatitis B virus by hepatitis B virus S genemodified dendritic cells

作　　者：雷春亮[1] 黄呈辉 杨湛[1] 唐小平[1] 

机构地区：[1]广州市第八人民医院 [2]深圳市宝安血站

出　　处：《中华实验和临床病毒学杂志》2007年第2期108-110,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：广东省自然基金项目（04003685）;广州市科技计划资助项目（2003J1-C0231）;广州市肝病重点专科重点项目（2005ZDi-05）

摘　　要：目的分析腺病毒载体介导HBVS基因修饰树突状细胞（DCs）能否诱导抗HBV特异性CTL反应。方法制备携带HBVS基因的重组腺病毒，分别转染外周血诱导培养的DCs，观察腺病毒转染DCs效率和DCs中HBV抗原的表达；混合淋巴细胞反应测定HBV抗原基因修饰DCs刺激同种异体T淋巴细胞增殖能力；乳酸脱氢酶释放法检测特异性CTL细胞对HepG2 22．1．5靶细胞的杀伤能力。结果腺病毒载体能够高效介导HBVS基因在DCs中表达，且DC细胞形态完整；HBVS基因修饰DCs具有刺激同种异体T细胞增殖能力，同时能诱导抗HBV特异性CTL反应。结论HBVS基因修饰DCs疫苗具有增强抗HBV特异性CTL效应的能力，可能发展为一种新型抗病毒疫苗。Objective To explore whether hepatitis B virus （HBV） S gene-modified dendritic cells （DCs） might induce a specific cytotoxic T lymphocyte （CTL） response. Methods The recombinant adenoviruses carrying HBsAg genes were prepared and used to transfect DCs generated from cord blood. The efficacy of transfection was observed through the expression of enhanced green fluorescent protein （EGFP） in DCs and the expression of HBsAg was detected by ELISA. HBV S gene-modified DCs were co-cultured with T cells from cord blood and T cells stimulating activities were detected using mixed lymphocyte reaction （MLR）. The CTL assay was carried out to assess the ability of CTL lines to lyse target cells of HepG2 22.1.5 by measuring lactate dehydrogenase （LDH） release. Results The results showed that HBV S genes were expressed in DCs with high efficacy by recombinant adenoviral vector. DCs had a normal shape after transfection. The result of MLR showed that HBV S gene-modified DCs could effectively stimulate naive T cells to proliferate. The induced specific CTL lines could lyse target cells of HepG2 22.1.5. Conclusions HBV S gene-modified DCs enhanced the function to induce a specific CTL effect, showing its promise for developing anti-viral vaccine in future.

关 键 词：肝炎表面抗原 乙型 树突细胞 T淋巴细胞 细胞毒性 腺病毒科 

分 类 号：R686[医药卫生—骨科学]