谷氨酰胺防治急性肝衰竭大鼠肠道细菌移位实验研究  

Experimental study on intestinal bacterial translocation in acute hepatic failure in rats

作　　者：刘寿荣[1] 娄国强[1] 黄文豹[1] 

机构地区：[1]杭州市第六人民医院,浙江310014

出　　处：《中华实验和临床病毒学杂志》2007年第2期126-128,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：杭州市医药卫生科技计划项目（20038038）

摘　　要：目的探讨谷氨酰胺（Gln）对急性肝衰竭（AHF）大鼠肠道细菌移位防治作用及机制。方法SD大鼠随机分成4组，对照组（A组），防治组（B组），治疗组（c组），模型组（D组）。B组、c组、D组腹腔注射D．氨基半乳糖（GaIN）建立急性肝功能不全大鼠模型。A组及D组予生理盐水灌胃。B组造模前两天予Gln灌胃防治，C组造模后一天Gln灌胃治疗。4d后处死动物。进行肝脏病理评分；观察肠系膜淋巴结细菌移位及平均组织含菌量、肠组织学改变；测量回肠绒毛高度和隐窝深度；检测血浆二胺氧化酶含量。结果B组和C组肝脏病理评分明显低于D组。B组肠系膜淋巴结细菌移位率低于D组，有显著性差异。B组、C组的血浆二胺氧化酶含量低于D组。B组显著低于C组。B组及C组的回肠绒毛高度和隐窝深度明显高于D组。B组明显高于C组，差异显著。结论谷氨酰胺可改善AHF大鼠的肠道黏膜屏障功能，减少AHF大鼠的肠道细菌移位的发生。Objective To investigate if glutamine（Gln） reeduce intestinal bacterial translocation in acute hepatic failure（AHF）iu rats and its mechanisms. Methecls Acute hepatic failure model in rat was established by intraperitoneal injection of galatosamine. The rats were randomly divided into 4 groups： the normal control group （A）, prevention and treatment group （B） , treatment group （C） , and model group （D） . The rats in groups A and D were fed with normal saline. Two days before intraperitoneal injection, the rats in group B were fed with Gin and those in group C were fed with Gin 24 hours after injection. After 4 days of treatment, the rats were sacrificed and pathological scores of liver were assessed. The percentage of intestinal bacterial transloaction and bacteria in mesenteric lymph nodes （MLN） were measured. The villus height, crypt depth of ileum mucosa were analyzed. The levels of serum diamine oxidase （DAO） were measured. Results The liver pathological scores of groups B and C were significantly lower than those of group D. The frequency of the bacteria found in MLN was significantly lower in group B compared with group D. The levels of DAO in blood were significantly lower in groups B and C than that of group D, and the level was significantly lower in group B than in group C. The villus height and crypt depth of the mucosa were significantly greater in group B and group C than in group D, and greater in group B than in group C. Conclusion The results of the present study show that Gin can reduce the occurrence of the intestinal bacterial translocation in AHF in rats by improving the function of intestinal barrier.

关 键 词：谷氨酰胺 肝功能衰竭 肠道细菌移位 

分 类 号：R686[医药卫生—骨科学]