NucliSens HIV-1QT和Amplicor HIV-1 monitor 1．5方法测定HIV-1病毒载量的比较研究  

Comparison of NucliSens HIV-1 QT and Amplicor HIV-1 monitor 1.5 in detecting HIV-1 viral load

作　　者：潘品良[1] 许文燕[1] 姚均[1] 陶晓霞[1] 裴丽健[1] 蒋岩[1] 

机构地区：[1]中国疾病预防控制中心性病艾滋病预防控制中心参比实验室,北京100050

出　　处：《中华实验和临床病毒学杂志》2007年第2期177-179,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：克林顿基金中国艾滋病卫国际双便函项目资助（2004）649

摘　　要：目的进行NuchSens HIV-1 QT和Amplicor HIV-1 monitor 1．5检测相同临床样品HIV-1病毒载量值之间的比较研究。方法收集临床样本82份，使用两种方法测定病毒载量，对病毒载量值进行统计分析。结果未测到核酸的和两种方法病毒载量对数值之差小于0．5的占88．9％；用△log10 VL〈0．5的56份样本统计两种方法的相关性，相关系数为0．956。结论NucliSens HIV-1 QT和Amphcor HIV-1 monitor 1．5两种方法测定的HIV病毒载量值有很好的相关性。Objective To compare the results of detecting HIV-1 load by using NucliSens HIV-1 QT and Amplicor HIV-1 monitor 1.5 assays. Methods Eighty-two clinical samples were collected and HIV viral load was determined with the above-mentioned two methods. Results The number of samples in which values obtained by ＂NucliSens HIV-1 QT and Amplicor HIV-1 monitor 1.5 differed by 〈 0.5 log10 RNA copies/ml and in which the viral load was undetectable accounted for 88.9% methods was 0.956 in 56 samples of ,△log10 EL 〈 0 with the two methods are highly comparable. of the measures. The correlation coefficient between the two 5. Conclusion The results of HIV-1 viral load determination with the two methods are highly comparable.

关 键 词：HIV-1 病毒载量 逆转录聚合酶链反应 核酸扩增技术 

分 类 号：R686[医药卫生—骨科学]