灯盏花素大鼠在体肠吸收动力学研究  

Absorption kinetics of breviscapine in intestine of rats in situ

作　　者：许英爱[1] 范国荣[1] 高申[1] 洪战英[1] 

机构地区：[1]第二军医大学药学院,上海200433

出　　处：《中草药》2007年第7期1036-1039,共4页Chinese Traditional and Herbal Drugs

基　　金：上海市药物(中药)代谢研究技术平台建设(03JC14005)

摘　　要：目的研究灯盏花素在大鼠肠道的吸收动力学。方法进行大鼠在体肠循环灌流肠吸收实验,HPLC法测定灯盏花乙素,研究灯盏花素在小肠和结肠的吸收情况,并分别考察不同质量浓度和不同pH对小肠吸收的影响。结果胆管结扎与胆管不结扎的实验组之间,吸收速率常数(ka)和吸收百分率均有显著性差异,在小肠和结肠的ka分别为(0.1071±0.0130)和(0.0707±0.0089)h-1;灯盏花素在不同质量浓度下,未发现饱和现象,其ka几乎保持不变,在pH6.0～7.4,灯盏花素的吸收不受pH的影响。结论灯盏花素在小肠的吸收多于在结肠的吸收,其吸收机制为被动扩散,吸收过程为一级动力学过程,提示灯盏花素可以被制成口服缓释剂型。Objective To investigate the intestinal absorption kinetics of breviscapine in rats. Methods The intestinal absorption in small intestine and colon of rats in situ was investigated using circular perfusion and HPLC methods. The in situ absorption of scutellarin in the small intestine was studied and the effects on the intestinal absorption were observed at the various concentrations and different pH values using the same methods. Results The results showed that there were significant differences in both absorptive percentage and absorption rate constant （ka） in the small intestine between the experimental groups of rats with and without bile duct being ligated. The absorption rate constants in small intestine and colon were （0. 107 1±0. 013 0） and （0. 070 7±0. 008 9） h^-1, respectively. No saturation phenomenon occurred and k. was almost kept unchanged at different concentrations of breviscapine. The absorption of breviscapine was not influenced by pH values ranging from 6.0 to 7.4. Conclusion The results indicates that breviscapine is absorbed more in small intestine than in colon. The absorption of breviscapine complied with the passive diffusion mechanism and first order kinetics. In conclusion, these results suggest that breviscapine could be prepared in oral sustained-release dosage form.

关 键 词：灯盏花素 在体 肠吸收 

分 类 号：R285.61[医药卫生—中药学]