不同光照强度对虎杖愈伤组织的影响  

Effect of different light intensity on Polygonum cuspidatum callus

作　　者：文涛[1] 梁莉[1] 曾杨[1] 喻晓[1] 

机构地区：[1]四川农业大学农学院,四川雅安625014

出　　处：《中国中药杂志》2007年第13期1277-1280,共4页China Journal of Chinese Materia Medica

摘　　要：目的：研究光照强度对虎杖愈伤组织的诱导形成及其白藜芦醇含量的影响。方法：分别以虎杖茎段、叶片为外植体,在不同光照强度下诱导和培养愈伤组织,对愈伤组织生长状况进行观察统计,HPLC测定其中白藜芦醇的含量。结果：光强为1340～1560 lx条件下诱导的茎段愈伤组织诱导形成较早,生长速度适宜,维持时间长,且其白藜芦醇质量分数最高,鲜茎中为18.350μg·g^-1干茎中为140.074μg·g^-1高于同期野生品。结论：不同光照强度下,虎杖愈伤组织的诱导和形态有差别,适当的光照强度能促进虎杖愈伤组织中白藜芦醇的合成。Objective： To study the effect of light intensity （LI） on the callus induction and resveratrol content of Polygonum cuspidatum. Method： Taking leaves and stem segment of P. cuspidatum as explants, the callus was inducted and cultured under different LI. The growth status of callus was observed and analyzed. The content of resveratrol was determined by HPLC. Result： The formation and growth of the stem segment callus were optimal under low light treatment （ 1 340～1 560 lx）. The resveratrol content was 18.350 μg·g^-1 in fresh stem segment and 140.074 μg·g^-1 in dry stem segment, which was two times higher than that of wild P. cuspidatum. Conclusion： Introduction and status of P. cuspidatum callus were obvious diverse under different LI, the resveratrol content of P. cuspidatum was enhanced under appropriate LI.

关 键 词：光照强度 虎杖 愈伤组织 白藜芦醇 

分 类 号：S567.239[农业科学—中草药栽培]