大孔吸附树脂分离纯化菝葜总黄酮  被引量:4

Study on separation and purification of total flavones from Smilax china by macroporous absorption resin

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作  者:李文霞[1] 颜彦[2] 叶晓川[2] 杨祥良[2] 

机构地区:[1]华中科技大学化学系,湖北武汉430074 [2]华中科技大学生命科学与技术学院,湖北武汉430074

出  处:《中国中药杂志》2007年第13期1292-1295,共4页China Journal of Chinese Materia Medica

摘  要:目的:研究大孔吸附树脂富集、纯化菝葜总黄酮的工艺条件及参数。方法:以总黄酮收率为指标,对工艺条件进行了筛选。结果:6种大孔吸附树脂和1种聚酰胺中,D140型树脂具有最佳的吸附及洗脱参数,其静态吸附容量以干树脂计为14.87 mg·g^-1。最佳吸附条件:药液质量浓度为0.5 g·mL^-1(以生药量计),吸附流速为2 BV.h-1,吸附量为18 BV;洗脱最佳条件:以10 BV蒸馏水、5 BV的20%乙醇依次洗脱,弃去洗脱液;再用5~10BV,60%乙醇以流速1 BV.h-1进行洗脱,总黄酮的收率为84.72%。结论:D140型树脂综合性能较好,适于菝葜总黄酮的分离纯化。Objective: To investigate the technological parameters of the purification process of total flavones from Smilax china with macroporous absorption resin. Method: The technical process for purification of total flavones with the optimum macroporous absorption resin was screened by yield of total flavones product. Result: The D140 macroporous absorption resin had the best separating efficiency when the flavones content in the liquid was 0. 5 g·mL^-1 equivalent to raw material, the volume of drug 18 BV (resin bad volume) with the adsorption-power 2 BV·h^-1, and the volume of 60% (mL·mL^-1) ethanol as eluant 5~10 BV (resin had volume) with desorption-power 1 BV·h^-1. The obtained flavones product has total flavones recovery rate of 84.72%. Conclusion: The treatment of regenerated resin is easy, this method is advisable.

关 键 词:大孔吸附树脂 菝葜总黄酮 分离纯化 

分 类 号:R284.1[医药卫生—中药学]

 

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