甜玉米成熟胚愈伤组织的诱导及植株再生特性的评价  

Characterization of Embryogenic Callus Induction and Plant Regeneration of Sweet Corn

作　　者：陈莉[1] 杨晋彬[1] 罗玉明[1] 阮元元[1] 张永霞[1] 曹俊梅[2] 窦秉德[1] 

机构地区：[1]淮阴师范学院植物生物技术研究所,江苏淮安223300 [2]新疆农业大学农学院,新疆乌鲁木齐830052

出　　处：《江苏农业学报》2007年第3期184-188,共5页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省高校高新技术产业化项目(JH03-003)

摘　　要：以45种基因型甜玉米的成熟胚为外植体材料,分析出愈率、愈伤组织增殖量、芽点数与植株再生的关系。结果表明,出愈率与再生能力有较大的相关性,愈伤组织增殖量、芽点数次之。利用主成分坐标分析并在此基础上对不同基因型甜玉米的再生能力进行聚类,并按欧氏距离将45种不同基因型玉米划分为2个大组群。通过对不同基因型玉米成熟胚培养特性的分析,探讨了各指标对于植株再生的意义,从供试材料中筛选出了愈伤组织诱导率和绿苗分化率均较高的材料,如选系B12-2-2和A50。The embryogenic calli induced from the mature embryos of forty-five genotypes of sweet corn varieties were treated as materials to analyze the relationship between plant regeneration and callus induction rate, callus proliferation and bud points. Callus induction rate correlated with plant regeneration more closely than the latter two factors according to the experimental results, Forty-five genotypic sweet corns were classified into two groups in Euclid space based on the clustering of the plant regeneration of the embryos using the analysis of principle components. Sweet corn line（variety） B12-2-2 and A50 were identified with higher performance in callus induction and plant regeneration.

关 键 词：甜玉米 成熟胚 愈伤组织诱导 植株再生特性 

分 类 号：S513.035.3[农业科学—作物学]