菠菜和黄瓜捕光叶绿素a/b蛋白质复合体的二维结晶  

Two dimensional Crystallization of The Light harvesting Chlorophyll a/b Protein Complexes of Spinach and Cucumber

作　　者：张兴[1,2,3] 徐伟[1,2,3] 娄世庆 王可玢[1,2,3] 黄有国 匡廷云[1,2,3] 

机构地区：[1]中国科学院北京电子显微镜实验室 [2]中国科学院生物物理研究所 [3]中国科学院植物研究所

出　　处：《电子显微学报》1997年第3期318-322,共5页Journal of Chinese Electron Microscopy Society

基　　金：国家自然科学基金

摘　　要：膜蛋白的二维结晶化是蛋白质电子晶体学解析膜蛋白三维结构的基础。我们用ｂａｔｃｈ方法实现了去垢剂溶解的黄瓜和菠菜的捕光叶绿素ａ／ｂ蛋白质复合体（ＬＨＣ－Ⅱ）的二维结晶，获得了质量良好的二维晶体。其中黄瓜ＬＨＣ－Ⅱ晶体面积可达５－１０μｍ以上，菠菜ＬＨＣ－Ⅱ晶体面积为２－４μｍ，晶体有序性良好，适用于较高分辨率电子晶体学结构研究。文章还讨论了黄瓜、菠菜以及豌豆的ＬＨＣ－Ⅱ二维晶体生长条件的异同。Two dimensional crystallization of membrane proteins is the base on which their three dimensional structure are solved by electron crystallography.Two dimensional crystals of the light harvesting chlorophyll a/b protein complexes (LHC Ⅱ) of cucumber and spinach,which have the size ranging 5_15μm for cucumber LHC Ⅱ,2—4μm for spinach LHC Ⅱ and well ordered,have been grown by the batch method.The conditions growing the crystals of LHC Ⅱ have been compared between pea,cucumber and spinach.Some factors affecting the qualities of the two dimensional crystals have also been discussed.

关 键 词：捕光叶绿素 蛋白质复合体 二维结晶 菠菜 黄瓜 

分 类 号：S636.101[农业科学—蔬菜学] S642.201[农业科学—园艺学]