云南紫稻细胞质无花粉型三系及杂种F_1的AFLP指纹图谱的构建与分析  

Construction of AFLP Fingerprinting and Comparative Analysis Between Three Lines of Cytoplasmic Non-Pollen Type in Yunnan Purple Rice and Their Hybrid F_1

作　　者：胡侃[1] 余夏夏[1] 余金洪[1] 丁毅[1] 

机构地区：[1]武汉大学植物发育生物学教育部重点实验室/生命科学学院,湖北武汉430072

出　　处：《中国水稻科学》2007年第4期345-349,共5页Chinese Journal of Rice Science

基　　金：国家自然科学基金资助项目(30571143);湖北省科技攻关项目(2006AA201C04)

摘　　要：利用AFLP分子标记对云南紫稻细胞质无花粉水稻三系及杂种F1进行了指纹图谱的构建与比较分析。从14对引物中筛选出8对具有明显多态性的引物。天香018(天香A×珞恢018)材料组(不育系、保持系、恢复系及杂种F1)共扩增出179条带,其中有52条多态性谱带,占总带数的29.05%,平均每对引物可以扩增出6.50条多态性谱带;天香806(天香A×珞恢806)材料组(不育系、保持系、恢复系及杂种F1)共扩增出177条带,其中有50条差异带,占总带数的28.25%,平均每对引物可以扩增出6.25条多态性谱带。特别明显的是在引物对E-ATG/M-CAC的扩增中,在AFLP指纹图谱中出现两条特征带(a带和b带),为保持系所特有,能够明显区别于不育系、恢复系和F1。在另外一些引物对中也观察到不育系与保持系之间的差异带。An AFLP fingerprinting was constructed to conduct comparative analysis on three lines of cytoplasmic nonpollen type in Yunnan purple rice and their hybrid F1. Eight of fourteen random primer pairs displayed significant polymorphisms. In Tianxiang 018（Tianxiang A× Luohui 018） group, including cytoplasmic male sterile line, maintainer line, restorer line and F1, 179 bands were amplified, 52 of which were polymorphic （29.05 %）; in Tianxiang 806 （Tianxiang A × Luohui 806） group （cytoplasmic male sterile line, maintainer line, restorer line and F1 ）, 177 bands were amplified, 50 of which were polymorphic （28.25 %）. Particularly, two specific bands amplified by the primer pair E-ATG/M-CAC were found only in the maintainer line and could obviously distinguish maintainer line from the cytoplasmic male sterile（CMS） line, restorer line and F1 respectively. Some characteristic bands between CMS line and its maintainer line were also observed in other primer pairs, and these would be valuable for identification and utilization of cytoplasmic non-pollen CMS in Yunnan purple rice as well as for further study on mechanism of CMS in hybrid rice.

关 键 词：细胞质雄性不育系 细胞质无花粉型雄性不育 紫稻 扩增片段长度多态性 指纹图谱 杂交水稻 

分 类 号：S511[农业科学—作物学]