一个除草剂安全剂AD-67诱导的水稻基因片段的克隆和分析  

Identification of a Herbicide Safener AD-67 Inducible cDNA in Rice

作　　者：殷得所[1] 孙小琼 李科[1] 王世全[1] 邓其明[1] 李平[1] 

机构地区：[1]四川农业大学水稻研究所 [2]四川农大高科农业有限责任公司,四川成都611130

出　　处：《中国水稻科学》2007年第4期439-442,共4页Chinese Journal of Rice Science

基　　金：国家高技术研究发展计划(863计划)资助项目(2003AA212030);教育部长江学者和创新团队发展计划资助项目(IRT0453)

摘　　要：为寻找化学诱导表达基因,利用mRNA差异显示方法,从除草剂安全剂AD-67(苯叉酰胺)诱导处理的水稻品种9311的幼苗中筛选分离得到1个诱导表达增强的cDNA片段。该片段在处理后6h表达明显增强,直到第5天仍然有表达。从poly-gel上回收片段并测序,核酸同源序列分析显示它与水稻多个EST和cDNA序列一致性达到100%,氨基酸序列分析表明它与水稻、四膜虫、隐球酵母等真核生物的Yippee基因相似性达60%～84%。序列经延伸得到831bp的cDNA,由此设计引物并进行RT-PCR分析,结果说明该基因在除草剂安全剂AD-67诱导后表达增强。A safener AD-67 inducible cDNA was identified in an indica rice variety 9311 by mRNA differential display. The transcript increased 6 h after the rice seedlings were sprayed with the safener solution and 5 days later, the expression could still be detected. The fragment was recycled from the poly-gel and sequenced, and homologous analysis revealed the cDNA was 100% identical to some ESTs and cDNAs in rice data base, and showed 60%- 84 % homologous to the gene Yippee in several eukaryotes in amino acid sequence. The fragment was extended to a 831 bp cDNA, a primer was designed and RT-PCR analysis supported the induction result.

关 键 词：水稻 化学诱导 差异显示 苯叉酰胺 Yippee基因 

分 类 号：S511[农业科学—作物学]