溶剂萃取法分离水溶性甘草黄酮  

Separation of Hydrophilic Licorice Flavonoid by Solvent Extraction Method

作　　者：田庆来[1] 谢渝春[1] 张波[1] 刘会洲[1] 

机构地区：[1]中国科学院过程工程研究所分离科学与工程实验室

出　　处：《过程工程学报》2007年第3期496-500,共5页The Chinese Journal of Process Engineering

摘　　要：以三烷基氧化膦(Trialkylphosphine Oxide,TRPO)石油醚溶液为萃取有机相,从甘草浸提液中对水溶性甘草黄酮进行了萃取分离.正交实验表明,TRPO浓度是影响总黄酮萃取的显著因素,相比(A/O)的影响次之,pH值的影响最小.在pH5～8的范围内总黄酮萃取率随pH值升高而逐渐下降;在pH5～6的范围内甘草酸的萃取率随pH值升高迅速降低,在pH6以上几乎降为0;总黄酮萃取率随相比的增大而减小,随萃取剂浓度的增大迅速提高;总黄酮的萃取率随温度的升高而下降,说明萃取黄酮的反应是放热反应.TRPO萃取甘草甙的萃合比为3.通过溶剂萃取方法可实现水溶性甘草黄酮和甘草酸的分离.Using the mixture of trialkylphosphine oxide （TRPO） and petroleum ether as extractive organic phase, the extraction of hydrophilic flavonoid from the aqueous leaching solution of licorice was carried out. Orthogonal tests showed that the concentration of TRPO was the most remarkable factor for extracting hydrophilic flavonoid, then the phase ratio and the smallest was pH value. It was also found that the extraction rate of total flavonoid （TF） decreased with increasing pH value in the range from 5 to 8. The extraction rate of glycyrrhizic acid rapidly decreased with increasing pH value in the range from 5 to 6, and it almost approached zero above pH 6. The extraction rate of TF rapidly increased with the increase of the concentration of TRPO and decreased with the increase of phase volume ratio （A/O）. It decreased with the increase of the system temperature, and the reaction of the extracting TF was exothermic. The ratio of TRPO to solute in extractive complex was 3. The separation between hydrophilic licorice flavonoids and glycyrrhizic acid in the aqueous leaching solution could be achieved by solvent extraction method.

关 键 词：甘草黄酮 甘草酸 萃取 分离 正交实验 

分 类 号：TQ460.6[化学工程—制药化工]