Aβ损伤后大鼠SYP和MAO的变化及加减地黄饮子的干预作用  

Changes of SYP Expression and MAO activity in Brain after Aβ_(140) Treated rats and Effect of Modified Decoction of Rehmanniae

作　　者：牛英才[1] 李鸿梅[1] 兴桂华[1] 张晓杰[1] 王建明[2] 谢宁[2] 

机构地区：[1]齐齐哈尔医学院医药科学研究所,黑龙江齐齐哈尔161042 [2]黑龙江中医药大学中医药研究院,黑龙江哈尔滨市150040

出　　处：《四川中医》2007年第7期17-19,共3页Journal of Sichuan of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30472128);黑龙江省博士后资助经费项目(LBH-Z05239)

摘　　要：目的:观察β-淀粉样蛋白1-40(Aβ1-40)大鼠海马双侧注射后大脑组织突触素(synaptophysin,SYP)表达和单胺氧化酶(monoamine oxidase,MAO)活性的变化及加减地黄饮子的干预作用。方法:采用大鼠海马立体定向注射凝聚态Aβ1-40诱导老年性痴呆(Alzheimer s disease,AD)动物模型。采用免疫印迹(western blotting)方法检测大脑组织SYP的表达,通过紫外分光光度法检测大鼠大脑组织MAO的活性。结果:模型组大鼠大脑组织SYP蛋白表达相对值(0.508±0.187)较假手术组(0.915±0.069,P<0.05)明显降低,而加减地黄饮子(0.833±0.077,P<0.05)可上调SYP的表达。模型组大鼠脑组织MAO活性(7.88±0.76U/h/mg/prot)较空白对照组(5.17±0.33U/h/mg/prot,P<0.05)明显升高,而这种升高可被加减地黄饮子(7.04±0.72U/h/mg/prot)抑制。结论:加减地黄饮子通过上调SYP蛋白的表达和抑制MAO活性而发挥保护Aβ对大鼠脑神经细胞的损伤。Objective ： To observe the effects of modified decoction of Rehmanniae on the expression of SYP protein and MAO activity in AD rats induced by amylold-β1-40 injection into brain. Methods： AD model rats was induced by amyloid-β1-40 sterotaxis injection into the bilateral Hippocampus. Modified decoction of Rehmanniae was given to AD rats by oral administration for 28 days. Expression of SYP protein in rat brain was determined by western blotting method. The activity of brain tissue MAO was detected by ultraviolet-spectrophotometry. Results： The western blot analysis showed that the levels of SYP expression in the model group （0.508 ± 0.187） was significantly lower than that in the sham operation group （0.915 ± 0.069） （ P 〈 0.05）, and the modified decoction of Rehmanniae regulated up the expression of SYP （0.833 ± 0.077） （P 〈 0.05）. Uhmviolet-spectrophotometry analysis showed that the MAO activity in the model group [. （7.88 ± 0.76） U/ （h/mg/prot） ] was significantly higher than that in the blank group [ （5.17 ± 0.33） U/ （h/mg/ prot） ] （ P 〈 0.05）, and the modified decoction of Rehmanniae inhibited the increase of MAO activity [ （7.04 ± 0.72） U/ （h/mg/ prot） ] （ P 〈 0.05 ）. Conclusion： Modified decoction of Rehmanniae can protect brain neurons injured from Aβ1-40 through up-regulating the expression of SYP protein and decreasing MAO activity.

关 键 词：阿尔茨海默氏病 加减地黄饮子 突触素 单胺氧化酶 

分 类 号：R256.33[医药卫生—中医内科学]