麦洼牦牛血红蛋白α链基因的克隆测序  

Cloning and Sequencing of α Chain Gene of Hemoglobin in Yak

作　　者：周静[1] 郑玉才[1] 金素钰[1] 陈炜[1] 文勇立[1] 苏永杰[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041

出　　处：《西北农业学报》2007年第4期63-66,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：四川省青年科技基金项目(No.05ZQ026-024)

摘　　要：从麦洼牦牛脊髓中提取总RNA,利用RT-PCR扩增血红蛋白α链基因,获得了532bp的cDNA,连接到PMD-18载体上,然后转化TOP-10感受态细胞进行克隆测序。结果显示,牦牛血红蛋白α链基因与普通牛相比存在2个碱基差异,并导致血红蛋白α链71位的1个氨基酸改变。此外,还用电泳方法研究了血红蛋白在新生犊牦牛中的表达。Total RNA was extracted from the spinal cord of Maiwa yak, and RT-PCR was carried out to amplify hemoglobin α chain gene. The amplified 532bp cDNA was ligated to PMD-18 vector, transformed to TOP-10 competent E. coli cells and sequenced. The sequence of yak hemoglobin α chain gene has two nucleotide differences with that of bovine, and result in one amino acid mutation at position 71 of hemoglobin α chain. Expression of hemoglobin was studied in newborn yak calves by electrophoresis.

关 键 词：牦牛 血红蛋白基因 克隆 测序 

分 类 号：S823[农业科学—畜牧学]