农杆菌介导的普利安娜百合鳞茎直接分化受体系统的建立  

Construction of the Bulblet Direct Differentiation Acceptor System of Lilium Pollyanna by Agrobacterium-mediated

作　　者：赵欢蕊[1] 刘雅莉[1] 王跃进[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《西北农业学报》2007年第4期142-147,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然基金资助项目(30371013)

摘　　要：以亚洲百合"普利安娜"为材料,通过不定芽诱导、植株再生及抗生素敏感性试验,建立了百合直接分化受体系统。结果表明:鳞片和试管苗鳞片叶不定芽分化培养基均以MS+BA2.0 mg/L+NAA0.2 mg/L为最佳;试管苗小叶柄分化不定芽培养基以MS+BA1.0 mg/L+NAA0.3 mg/L为宜;1/2MS+IBA0.5 mg/L+90 g Sucrose+暗培养1个月后再光照培养可诱导形成试管苗鳞茎;生根的适宜培养基为1/2MS+IBA0.5mg/L+0.1%活性炭;卡那霉素筛选浓度鳞片为150 mg/L,鳞片叶为100 mg/L。With the Asiatic hybrids, Lilium Pollyanna as the research material, a direct differentiation acceptor system was developed by adventitious buds induction, plant reproduction and antibiotics sensitive experiments. The results showed that the optimum culture medium for the scales and the scale leaves of the test-tube plantlets to differentiate adventitious buds were MS＋BA2.0 mg/L＋NAA0.2 mg/L; The suitable culture medium for the petioles of the test-tube plantlets to differentiate adventi- tious buds was MS＋BA1.0 mg/L＋NAA0.3 mg/L; The culture medium for bulblet formation of the test-tube plantlets was 1/2MS＋ IBA0.5 mg/L＋ 90 g Sucrose＋ light culture after dark culture one month; The appropriate medium for rooting was 1/2MS＋IBA0.5 mg/L＋0.1 ~AC; The concentra- tion of kannamycin was 150 mg/L for the scales, and 100 mg/L for the scale leaves.

关 键 词：亚洲百合 农杆菌介导 遗传转化 直接分化受体系统 

分 类 号：S682.29[农业科学—观赏园艺]