补阳还五汤对脑出血大鼠脑内MMP-2表达的影响  

The effects of Buyang Huanwu Decoction on expressions of matrix metalloproteinase-2 in intracerebral hemorrhagic rat brains

作　　者：王玲[1] 唐涛[1] 罗杰坤[1] 黄菊芳[2] 杨期东[3] 黎杏群[1] 齐勇[1] 张花先[1] 刘小娟[1] 邢之华[1] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,湖南长沙410008 [2]长沙中南大学湘雅医学院神经生物学研究室 [3]长沙中南大学湘雅医院神经内科

出　　处：《中国老年学杂志》2007年第13期1257-1260,共4页Chinese Journal of Gerontology

基　　金：国家自然科学青年基金(30400581);中国博士后科学基金(2005038224);湖南省青年骨干教师培养对象经费(湘教通〔2005〕64号);湖南省重点学科建设经费(湘教通〔2001〕179号)

摘　　要：目的观察脑出血大鼠脑组织中基质金属蛋白酶-2(MMP-2)表达,探讨补阳还五汤治疗脑出血的作用机制。方法采用立体定向技术制作大鼠脑出血模型,对出血后2、4、7、14、21、28 d后各组脑组织进行MMP-2免疫组织化学染色,检测脑组织各区域MMP-2的阳性微血管数。结果正常组未见表达,假手术组皮质偶见MMP-2表达;模型组大鼠MMP-2呈双峰表达,在造模后4 d达最高峰(P<0.01),之后有所下降,至21 d仍有表达并再有小高峰出现(P<0.01);补阳还五汤组4 d时为表达低谷,并明显低于模型组(P<0.01),其后逐渐上升至高水平维持达2 w,其中7、14、21 d表达均高于模型组(P<0.05),28 d表达稍下降但仍高于模型组(P<0.01)。结论补阳还五汤可通过调节脑出血后MMP-2的表达,有利于微血管系统重建,改善出血后脑组织的微环境。Objective To investigate the mechanisms of Buyang Huanwu Decoction （BYHUD） by observing its effects on expressions of roatrix metalloproteinase-2 （MMP-2）in intracerebral hemorrhagic （ICH） rat brains. Methods ICH model was induced by injecting collagenase type Ⅶ stereotaxically into right globus pallidus. Sprague-Dawley rats were randomly divided into normal control group, sham operation group, model group and BYHUD-treated group. MMP-2 and MMP-2 positive microvessel count were detected on the 2nd, 4th, 7th, 14th,21th and 28th day after operation. The expressions of MMP-2 were evaluated by immunohistochemistry. Results Expression of MMP-2 was not observed in normal control group and observed occasionally in the cortex of sham operation group. In model group, the expression of MMP-2 was double hump and peaked on the 4th day （P 〈0. 01 ） ,then gradually decreased but peaked at 21th day （P 〈0. 01 ） again. In BY- HUD-treated group, expression of MMP-2 was lower than that in model group on the 4th day （P 〈 0. 01 ） , then increased and was higher than that in the model group during the 7th -21th day （P 〈0. 05）. It depressed on 28th day,but was still higher than that in the model group （P 〈 0. 01 ）. Conclusions BYHUD could regulate the expressions of MMP-2 in ICH rat brains, which might improve the microenvironment and promote the microvessel networks reconstruction after ICH.

关 键 词：补阳还五汤 脑出血 基质金属蛋白酶-2 微血管 大鼠 

分 类 号：R277.743[医药卫生—中医学]