高效液相色谱法测定安宫牛黄丸中栀子苷、黄芩苷和盐酸小檗碱的含量  

Simultaneous determination of geniposide,baicalin and berberine hydrochloride in Angong Niuhuang pill by RP-HPLC

作　　者：韩凤梅[1] 李宇[1] 李路军[1] 陈勇[1] 

机构地区：[1]湖北大学中药生物技术省重点实验室,湖北武汉430062

出　　处：《中国中药杂志》2007年第14期1406-1408,共3页China Journal of Chinese Materia Medica

基　　金：湖北省青年杰出人才科学基金(2002AC004)

摘　　要：目的：建立同时测定安宫牛黄丸中栀子苷、黄芩苷和盐酸小檗碱含量的反相液相色谱法。方法：采用Agilent ZORBAX SB—C18柱（4．6mm×250mm，5μm），乙腈-6mmol·L^-1 KH2PO4水溶液（pH4．6）梯度洗脱，检测波长240nm。结果：栀子苷、黄芩苷和盐酸小檗碱的线性范围分别为4．50—110．00，5．00—153．00，6．40—191．00mg·L^-1；质量检测下限分别为0．77，1．53，1.43ng；栀子苷的回收率为104．44％，RSD为1．79％；黄芩苷的回收率为96．98％，RSD为1．76％；盐酸小檗碱的回收率为101．08％，RSD为3．1％。结论：采用该方法同时测定安宫牛黄丸中栀子苷、黄芩苷和盐酸小檗碱含量具有简便、快速、准确等优点。Objective： A new method for the simultaneous quantitative determination of geniposide, baicalin and berberine hydrochloride in Angong Niuhuang pill using reversed phase high performance hquid chromatographic method had been developed. Method： The optimum chromatographic conditions were as follows： Agilent Zorbax SB - C18 column （4. 6 mm 250 mm, 5 m）, acetonitrile-H2O （containing 6 mmol · L^-1 KH2PO4, pH 4. 6） gradient elution; as a detection wavelength of 343 nm. Result： The calibration curves of geniposide, baicalin and berberine were linear at the ranges of 4.50-110. 00,5.00-153.00, 6. 40-191.00 mg · L^-1 , respectively. The limits of detection of the method were 0. 77 ng for geniposide, 1.53 ng for baicalin and 1.43 ng for berberine hydrochloride. The recoveries of the method were 104. 44% （ RSD 1.79% ） for geniposide, 96. 98% （ RSD 1.76% ） for baicalin, 101.08% （RSD 3.1% ） for berberine hydrochloride. Conclusion： This method had been successfully applied to determine the content of geniposide, baicalin and berberine hydrochloride in Angong Niuhuang pill.

关 键 词：HPLC 安宫牛黄丸 栀子苷 黄芩苷 盐酸小檗碱 

分 类 号：R286[医药卫生—中药学]