CX3C型趋化因子受体CX3CR1 mRNA在银屑病患者中的表达  

Expression of CX3C type chemokine receptor CX3CR1 mRNA in patients with psoriasis

作　　者：杨桂兰[1] 赵治华[2] 陈志强[3] 郑家润[3] 李新宇[3] 陈沄[3] 高纪伟[3] 徐兰芳[3] 唐美育[3] 

机构地区：[1]兰州军区兰州总医院皮肤科,兰州730050 [2]兰州军区兰州总医院专家组,兰州730050 [3]中国医学科学院皮肤病研究所,南京210042

出　　处：《中国麻风皮肤病杂志》2007年第7期557-560,共4页China Journal of Leprosy and Skin Diseases

基　　金：卫生部科技专项基金资助课题(981041)

摘　　要：目的:测定趋化因子受体CX3CR1 mRNA在银屑病患者中的表达水平及其与PASI之间的关系。方法:应用RT-PCR法检测了33例斑块状银屑病患者皮损处、非皮损处皮肤和淋巴细胞及其中16例治疗后患者淋巴细胞中CX3CR1 mRNA的表达水平,设30例健康人为对照组;将检测结果与PASI及皮损处与淋巴细胞中该受体的表达水平分别进行相关性分析。结果:CX3CR1mRNA表达水平:在银屑病治疗前与治疗后外周血淋巴细胞中分别为1.64±1.19与2.15±1.30,均明显高于对照组(0.72±0.72,P<0.001);在银屑病皮损处表皮中为1.33±0.40,明显高于对照组(0.89±0.26,P<0.001)及非皮损处皮肤(0.94±0.45,P<0.01);在银屑病皮损处真皮中为1.59±0.76,明显高于对照组(0.89±0.41,P<0.001)及非皮损处皮肤(1.21±0.53,P<0.01),并与PASI(r=0.601,P<0.001)及淋巴细胞中该受体的表达水平之间呈正相关(r=0.351,P<0.05),非皮损处真皮中CX3CR1 mRNA表达水平也高于对照组(P<0.05)。结论:CX3CR1可能主要通过参与活化、趋化淋巴细胞而在银屑病的发病机制及病理状态的维持中起到重要作用。ObjecTive： To investigate the expression of chemokine receptor CX3CR1 mRNA in patients with progressive plaque psoriasis and its correlation with psoriasis area and severity index （PASI）. Methods： Reverse transcriptase- polymerase chain reaction （RT- PCR） technique was applied to semi - quantitatively analyze CX3CR1 mRNA expression in epidermis and dermis from lesional and non - lesional skin, and peripheral blood lymphocytes in 33 patients with psoriasis vulgaris before treatment and 30 normal controls. Of the 33 patients the expression of chemokine receptor CX3CR1 mRNA in lymphocyte was measured in 16 patients with about 70% remission after treatment. The correlations between CX3CR1 mRNA values and PASI, and between the CX3CR1 mRNA values in lymphocytes and in epidermis and dermis were evaluated, respectively. Results： CX3CR1 mRNA levels in lymphocytes from pretreated and post- treated psoriatic patients were 1.64 ± 1.19 and 2.15 ± 1.30, respectively, both markedly higher than that in normal controls （0.72 ± 0.72, P 〈 0.001 ）. CX3CR1 mRNA level in psoriatic epidermis was 1.33 ±0.40, markedly higher than that in normal controls （0.89 ± 0.26, P 〈 0.001）and psoriatic non - lesional epidermis（0.94 ± 0.45, P 〈 0.01 ）. CX3CRlmRNA level in psoriatic lesional dermis was 1.59 ± 0.76, markedly higher than that in normal controls （0.89 ± 0.41, P 〈 0.001）and non - lesional dermis （ 1.21 ± 0.53, P 〈0.01）, and the CX3CRlmRNA level in non- lesional dermis was also higher than that in normal control （P 〈 0.05）. Positive correlations were noticed between CX3CR1 mRNA levels and PASI （r = 0.601, P 〈 0.001）, and the expression of CX3CR1 mRNA levels between dermis and lymphocytes （ r = 0. 351, P 〈 0.05）. Conclusion： The increased expression of CX3CR1 in psoriasis may be associated with migration and aggregation of lymphocytes in psoriatic lesion, suggesting that this chemokine receptor be involved in the pathogenesis of progressive plaque psoriasis.

关 键 词：银屑病 发病机制 受体CX3CR1 趋化因子 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]