申克孢子丝菌双相体外药物敏感试验研究  

Antifungal susceptibilities of Sporothrix schenckii in vitro

作　　者：赵景辉[1] 孙秋宁[1] 闫岩[1] 宿斌[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院皮肤科,100730

出　　处：《中国麻风皮肤病杂志》2007年第7期575-578,共4页China Journal of Leprosy and Skin Diseases

摘　　要：目的:研究双相真菌申克孢子丝菌分别在菌丝相和酵母相两种形态下对常用抗真菌药物的敏感性。方法:分别采用Rosco纸片扩散法及微量液基稀释法对临床分离的4株申克孢子丝菌的两种形态(菌丝相及酵母相)进行体外药敏试验。测定的抗真菌药物为:两性霉素B、伊曲康唑、氟康唑、特比萘芬。结果:除AMB二者一致外,其余各抗真菌药的MIC,菌丝相30℃孵育比酵母相35℃孵育高。结论:申克孢子丝菌的生长形态(菌丝相或酵母相)及孵育温度(30℃或35℃)对其药敏试验MIC值有明显影响。Objective： To determine the antifungal susceptibilities of dimorphic fungus Sporothrix schenckii. Methods： Four clinical isolates of Sporothrix schenckii were tested with amphotericin B, itraconazole, fluconazole and terbinafin. The susceptibility was measured by Rosco disk diffusion method. Results： The MICs were significantly lower for the yeast- like forms at 35℃ than for the mycelial forms at 30℃ for all antifungal agents, except for amphotericin B. Conclusion： The type of growth phase and the temperature of incubation have a significant influence on the MICs against Sporothrix schenckii.

关 键 词：申克孢子丝菌 双相真菌 药物敏感性 ROSCO纸片扩散法 微量液基稀释法 

分 类 号：R446.5[医药卫生—诊断学]