C57BL/6小鼠感染新生隐球菌对大脑皮质BDNF和bFGF表达的影响  

Effect of C57BL/6 mice infected with Cryptococcus neoformans on the expression of BDNF and bFGF in cerebral cortex

作　　者：王溪涛[1] 温海[1] 徐赤宇[2] 朱红梅[1] 

机构地区：[1]第二军医大学长征医院皮肤科 [2]沈阳军区第210医院皮肤科,大连116002

出　　处：《中国麻风皮肤病杂志》2007年第7期579-582,共4页China Journal of Leprosy and Skin Diseases

基　　金：国家自然科学基金资助项目(项目编号:30471566;30500441)

摘　　要：目的:观察C57BL/6小鼠感染新生隐球菌后对大脑皮质BDNF和bFGF表达的影响,并探讨BDNF和bFGF在新生隐球菌感染过程中的作用及其意义。方法:48只C57BL/6雌性小鼠随机分为对照组、环磷酰胺组和试验组,免疫组化法检测大脑皮质BDNF和bFGF的表达。结果:尾静脉注入新生隐球菌后6h大脑皮质查见新生隐球菌,2h大脑皮质BDNF的表达明显高于对照组和环磷酰胺组(P<0.01),0.5h大脑皮质bFGF的表达明显高于对照组和环磷酰胺组(P<0.01)。结论:新生隐球菌感染后大脑皮质BDNF和bFGF表达增高,提示BDNF和bFGF对新生隐球菌性脑膜炎具有修复作用,有望成为潜在的防治新生隐球菌性脑膜炎的药物。Objective： To observe the effect on the expression of BDNF and bFGF in cerebral cortex of C57BL/6 mice infected with Cryptococcus neoformans. Methods： Forty- eight C57BL/6 female mice were randomly divided into experiment group （received 10^6 cells of C. neoformans via the tail vein）, CTX group and controls. The expres- sion of BDNF and bFGF was detected by immunohistochemical technique. Results： Lots of C. neoformans were observed in cerebral cortex of experiment groups after 6 h. After 2 hours, the expression of BDNF in cerebral cortex of experiment group was significantly higher than control group and CTX group （ P 〈0.01）. After 0.5 hours, the expression of bFGF in cerebral cortex of experiment group was significantly higher than control group and CTX group （P 〈 0.01）. Conclusion： BDNF and bFGF may play a positive role in repairmen of cryptococcal meningitis and is potential to be used for the treatment of cryptococcal meningitis.

关 键 词：新生隐球菌 脑源性神经营养因子 碱性成纤维细胞因子 大脑皮质 新生隐球菌 性脑膜炎 

分 类 号：R519[医药卫生—内科学]