白塞病患者外周血单个核细胞分泌白细胞介素-10干扰素-γ水平的研究  

Study on level and significance of interleukin-10 and interferon-γ secreted by peripheral blood mononuclear cells in patients with Behcet′s disease

作　　者：张东霞[1] 邹顼雯[1] 罗东平[1] 

机构地区：[1]山西医科大学第一医院,030001

出　　处：《山西医药杂志》2007年第7期595-596,共2页Shanxi Medical Journal

摘　　要：目的通过检测白塞病(BD)患者外周血单个核细胞(PBMC)分泌白细胞介素-10(IL-10)、γ-干扰素(IFN-γ)水平,探讨T淋巴细胞亚群与BD发病的关系。方法采用酶联免疫吸附试验(ELISA)测定45例BD患者和20名健康志愿者PBMC培养上清IL-10和IFN-γ水平。结果①BD活动组PBMC自分泌IL-10水平明显升高,与非活动组、对照组比较差异有统计学意义(P<0.01);非活动组明显高于对照组(P<0.01)。②PBMC分泌IFN-γ水平3组(BD活动组、非活动组、对照组)比较差异无统计学意义(P>0.05)。BD患者PBMC分泌IL-10水平明显升高,增高程度与病情活动性相关,IFN-γ分泌水平无明显变化。结论PBMC分泌IL-10水平对BD诊断和病情活动性检测均有一定临床意义。Objective To investigate interleukin-10 （IL-10,Th2-type cytokine） and interferon-gamma （IFN-γ, Thl-type cytoklne） secreting capacity by peripheral blood mononuclear cells （PBMCs） in 45 inpatients with Behcet＇s disease（BD）and 10 healthy individuals,and try to understand the role of Th1/Th2 balance in the pathogenesis of BD. Methods Sandwich ELISA was used to measure IL-10 and IFN-7 level in the supematant of culture from PBMCs. Resuits The level of IL-10 produced spontaneously by PBMCs in active stage was significantly higher than that in static phase and healthy person （ P〈0.01 ）, There are significant difference between active and inactive patients （ P〈0.01 ）. The IFN-γ level was not statistically different between BE） patients and controls （P〉0.05）. Conclusion IL-10 may olav a significant role in the pathogenesis and disease activity of BD.

关 键 词：白细胞介素10 干扰素Ⅱ型 白塞病 

分 类 号：R597.9[医药卫生—内科学]