东北草莓×凤梨草莓种间杂种一代的细胞遗传学观察与RAPD分析  

Cytogenetics and RAPD Analysis of Interspecific Hybrids from the Cross of Fragaria mandschurica Staudt and F.ananassa Duch.

作　　者：马鸿翔[1] 陈佩度[2] 余桂红[1] 任丽娟[1] 

机构地区：[1]江苏省农业科学院农业生物遗传生理研究所,南京210014 [2]南京农业大学作物遗传与种质创新国家重点实验室,南京210095

出　　处：《园艺学报》2007年第3期597-604,共8页Acta Horticulturae Sinica

基　　金：江苏省自然科学基金项目(BK2001417)

摘　　要：以利用我国原产草莓二倍体野生种东北草莓与八倍体栽培种凤梨草莓栽培品种‘圣星’杂交,通过胚拯救获得的种间杂种为试材,经体细胞鉴定种间杂交后代为五倍体,育性差。叶柄茸毛和花梗茸毛与野生种母本东北草莓相近;花序着生高度部分与母本一致,部分与父本一致,各占一半;植株高度多数介于双亲之间,趋向于栽培品种。杂交F1代的花粉母细胞减数分裂观察表明,染色体构型为6·1Ⅰ+10·6Ⅱ+2·1Ⅲ+0·35Ⅳ,后期出现少量畸形多分孢子。RAPD分析表明,杂种后代具有亲本扩增片段,后代个体间存在遗传异质性。The interspecific hybrid was obtained from the cross of Fragaria mandschurica Staudt, a diploid species derived from Northeast China, and F. ananassa Duch. （2n = 8x = 56） ‘ Shengxing’ by using immature embryo culture technique. The hybrids were pentaploids with poor fertility. The morphological characteristics of the hybrids were observed and showed that they were intermediate between their parents in peduncle height and plant height, and similar to Fragaria mandschurica in hair on peduncle and petiole. The hybrids showed expected 35 chromosomes in somatic cells and had a meiotic chromosome association of 6. 1 Ⅰ ＋ 10. 6 Ⅱ ＋ 2. 1 Ⅲ ＋ 0. 35 Ⅳ with a few abnormal microspores occurring in tetrad phase. RAPD analysis showed that the hybrids among the interspecific possessed the specific bands of two parents, and the genetic diversities were found hybrid lines.

关 键 词：草莓 东北草莓 凤梨草莓 种间杂交 细胞遗传学 RAPD 

分 类 号：S668.4[农业科学—果树学]