板栗短雄花序异常死亡的超微结构观察  

Studies on the Ultrastructure of Abnormal Cell Death During Mutant Short Catkin Development in Chestnut

作　　者：张婧[1] 董清华[1] 杨凯[2] 曹庆芹[2] 冯永庆[1] 秦岭[1] 

机构地区：[1]北京农学院植物科学技术系,北京102206 [2]北京农学院生物技术系,北京102206

出　　处：《园艺学报》2007年第3期605-608,共4页Acta Horticulturae Sinica

基　　金：北京自然科学基金资助项目(6042004);北京市属市管高校人才强教计划项目;北京都市农业学科群资助项目(XK100190553)

摘　　要：板栗(Castanea mollissimaBl.)短雄花序发育过程中,花序上部变黄,弯曲,最后枯死脱落,而基部花序正常发育。利用透射电镜观察花序上部发育中的细胞超微结构发现:细胞呈现有序的死亡变化,细胞核内发生染色质凝聚,继而核仁消失,核质降解,核膜破裂,核解体;液泡内吞现象明显,形成很多小液泡;叶绿体和线粒体也逐渐解体;花序上部衰亡过程中所有细胞器在膜包裹下有序地降解,表现出植物细胞程序性死亡的典型形态学特征。During the development of short catkins mutant, the upper parts became yellow, curved, and finally dropped off gradually, while the basal catkins still developed normally. The ultrastructure changes of cells were observed during upper naturally degradation by using transmission electron microscope. The results exhibited orderly changes in death： the nucleus showed chromatin condensation, the nucleolus dissolved, karyoplasm degraded, the karyotheca burst and the nucleus disintegrated, the vacuole happened distinct swallow, and some formed into many small vacuoles, the chloroplast and mitochondrion disintegrated gradually. The morphological changes during the death of all the organelles within the cell showed the typical features of programmed cell death.

关 键 词：板栗 短雄花序 超微结构 细胞程序性死亡 

分 类 号：S664.2[农业科学—果树学]