白菜抗霜霉病基因的RAPD标记  

The RAPD Markers Linked to Downy Mildew Resistant Gene in Non-heading Chinese Cabbage (Brassica campestris ssp.chinensis Makino)

作　　者：冷月强 侯喜林[1] 史公军[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,南京210095

出　　处：《园艺学报》2007年第3期763-766,共4页Acta Horticulturae Sinica

基　　金：高等学校博士学科点专项基金项目(20030307021);国家‘863’项目(2006AA10ZIC9)

摘　　要：研究了与抗霜霉病基因紧密连锁的RAPD分子标记,利用白菜抗病品种014和感病品种010杂交的F2群体144个单株为材料,通过人工接种鉴定,采用高抗单株和高感单株分别构建抗、感病池,利用BSA法筛选了560条RAPD引物,其中引物AY12在抗感病池中扩增出多态性片段AY121238,通过F2单株验证后证明AY121238与抗霜霉病基因紧密连锁,其遗传距离为6·7cM。The objective of this study was to identify RAPD （ Random Amplified Polymorphic DNA） markers for downy mildew resistance in non-heading Chinese cabbage. RAPD-PCR （Polymerase Chain Reaction） analysis was conducted using BSA （Bulked Segregant Analysis） of a segregating F2 population which has 144 individuals from a cross between 010 （susceptible） and 014 （resistant）. After performing RAPD-PCR analysis with 560 arbitrary 10-mer primers and agarose-gel electrophoresis, only one RAPD marker AY121238 was identified to be linked to the resistant gene and was determined to be 6. 7 cM from the resistant gene.

关 键 词：白菜 霜霉病 RAPD标记 遗传距离 

分 类 号：S634.3[农业科学—蔬菜学]