几丁聚糖对氯化镉诱导HepG2细胞产生ROS水平的影响  

Effect of chitosan on the level of ROS in HepG2 cell induced by cadmium chloride

作　　者：谢虹[1] 王家春[1] 范冠宇[1] 吴志刚[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系,教育部环境与健康重点实验室,武汉430030

出　　处：《卫生研究》2007年第4期438-439,共2页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目(No.30371197)

摘　　要：目的研究不同浓度几丁聚糖对氯化镉诱导HepG2细胞产生活性氧(ROS)的清除作用。方法实验分设6组:正常对照组(仅含10%新生牛血清的培养液,无氯化镉和几丁聚糖)、20μmol/L氯化镉组、0.50mg/ml几丁聚糖组、20μmol/L氯化镉+几丁聚糖三个联合作用组(浓度分别为0.02、0.10和0.50mg/ml)。HepG2细胞处理时间为4h。以氯化镉诱导HepG2细胞产生ROS,运用DCFH-DA作为荧光探针,采用流式细胞检测技术检测几丁聚糖与氯化镉联合作用下HepG2细胞内ROS水平。结果20μmol/L氯化镉可使HepG2细胞内ROS水平显著增加(P<0.01);0.50mg/ml几丁聚糖处理HepG2细胞后,细胞内ROS水平较正常对照组略有降低,但并无显著性差异;与氯化镉染毒组相比,几丁聚糖和氯化镉联合作用组细胞内ROS水平随几丁聚糖浓度的增加,其DCF密度值由275.593逐渐降低到174.597,并且在中、高剂量几丁聚糖(0.1mg/ml和0.5mg/ml)和氯化镉联合作用组差异有极显著性(P<0.01)。结论几丁聚糖对HepG2细胞内由氯化镉诱导而产生的ROS水平具有明确的调节作用。Objective To study the effect of chitosan on ROS levels in HepG2 cell induced by cadmium chloride. Methods The experiments were divided into 6 groups： normal control group, cadmium chloride group（20μmol/L）, chitosan group（0.50mg/ml） and three combined treatment group（cadmium chloride 20μmol/L and chitosan 0.02mg/ml, 0.10mg/ml and 0.50mg/ml） . The HepG2 cells were treated by chitosan and cadmium chloride simultaneously for 4h. The fluorescence of 2′, 7′-dichlorofluorescin（DCF） was measured by flowcytometry as a mean of estimating the formation of reactive oxygen species （ROS）. Results In the comparison with normal control, the DCF intensity of chitosan group only exhibited somewhat decrease （P 〉 0.05） and significantly increased in cadmium chloride group（20μmol/L） （P 〈 0.01）. The DCF intensity decreased in all three combined groups were more higher thart those of cadmium chloride group, and when the concentration of chitosan improved from 0.01mg/ml to 0.50mg/ml, the DCF intensity decreased from 275.593 to 174.597 and the DCF intensity significant decrease was observed at concentration of 0.10mg/ml and 0.50mg/ml chitosan. Conclusion It was suggested that chitosan could antagonize the increase of ROS in HepG2 cell induced by cadmium chloride.

关 键 词：几丁聚糖 镉 活性氧 

分 类 号：R114[医药卫生—卫生毒理学] R151[医药卫生—公共卫生与预防医学]