γ-H2AX与DNA双链断裂关系的研究进展  

Association between γ-H2AX and DNA double-strand breaks

作　　者：宾萍[1] 郑玉新[1] 

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所,北京100050

出　　处：《卫生研究》2007年第4期520-522,共3页Journal of Hygiene Research

基　　金：国家973计划资助项目(No.2002CB512903);国家自然科学基金资助项目(No.30400348)

摘　　要：H2AX是组蛋白的一个种类,普遍存在于整个基因组中。据报道,DNA双链断裂后可诱导位于丝氨酸-139位C-端保守区域内的H2AX磷酸化形成γ-H2AX,在荧光显微镜下形成可见的γ-H2AX焦点。用特异性抗体的免疫荧光法检测γ-H2AX已成为测定DSB的金标准。目前,多种物理性、化学性和生物性的因素都能诱导形成γ-H2AX焦点。本文综述了不同因素诱导形成γ-H2AX的机制及其与DNA双链断裂之间的关系的研究进展。H2AX may be one family of histone and distributes among the genome. It is reported that DNA doublestrand breaks （DSB） could induce phosphorylation of H2AX at conservative C-terminal region of serine 139 and the formation of γ-H2AX, The γ-H2AX foci can be observed by the fluorescence microscope, An immunofluorescence assay with specific antibodies recognizing γ-H2AX could become the gold standard for detecting the DSB, It was indicated that some of physical, chemical, and biological factors could induce the formation of the γ-H2AX foci. In this paper, the advance of the studies of mechanism of γ-H2AX formation and the association between γ-H2AX and DSB were reviewed.

关 键 词：DNA损伤 Γ-H2AX DNA双链断裂 

分 类 号：Q523[生物学—生物化学]