超氧化物歧化酶对大鼠缺血骨骼肌钢泵、钙泵和细胞形态的影响  

EFFECTS OF SUPEROXIDE DISMUTASE ON Na^+-PUMP,Ca^(2+)-PUMP AND MORPHOLOGY OF THE ISOLATED RAT LIMB MUSCLES

作　　者：李继峰[1] 顾玉东[1] 袁伟[1] 钟慈声[2] 凌诒萍[2] 

机构地区：[1]上海医科大学华山医院手外科,200040 [2]上海医科大学基础医学院生物物理学教研室,200032

出　　处：《上海医科大学学报》1997年第2期103-106,共4页Journal of Fudan University(Medical Science)

基　　金：美国中华医学基金!CMB NO.90-528

摘　　要：研究超氧化物歧化酶对缺血骨骼肌和血管平滑肌的保护作用。应用大鼠离体肢体分别用超氧化物歧化酶（SOD）和生理盐水溶液灌注，保存0～48h，经2，4，8，16，24，48，72h取小腿三头肌进行Na＋－K＋－ATP醇和Ca2＋－ATP酶活性检测，并对此骨骼肌和股动脉平滑肌进行超微结构观察。结果：发现SOD灌注对缺血骨骷肌、股动脉平滑肌的酶活性及细胞形态都优于生理盐水灌注组（P＜0．01）。结论：结果表明SOD灌注时离体肢体的存活有保护作用。PURPOSE To study the effect of superoxide dimutase (SOD) on preservation of isolated rat limbs.METHODS Isolated rat hind were perfused with 0.9 % NaCl solution containing SOD and the control perfused with 0.9 % NaCl solution only. At different intervals after the perfusions, Na+-K+-ATPase and Ca2+-ATPase activities and cell ultrastructure of the triceps muscle and the femoral artery smooth muscle were observed.RESULTS It was showed that both the enzyme activities and the cell ultrastructure were preserved better in the limbs perfused with 0.9 % NaCl containing SOD than those in the limbs perfused without SOD.CONCLUSIONS The results suggest that SOD can preserve better the isolated limbs and prevent in from lesion caused by oxygen free radicals.

关 键 词：超氧化物歧化酶 钠泵 钙泵 骨骼肌 平滑肌 缺血 

分 类 号：R977.3[医药卫生—药品]