黄芩苷对人毛囊生长和毛乳头细胞分泌血管内皮生长因子的影响  

In vitro effects of baicalin on the growth of human hair follicles and secretion of vascular endothelial growth factor by human dermal papilla cells

作　　者：朱海琴[1] 范卫新[1] 张卉[1] 

机构地区：[1]南京医科大学第一附属医院皮肤科,210029

出　　处：《中华皮肤科杂志》2007年第7期416-418,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金(30571678)

摘　　要：目的探讨黄芩苷对体外培养的人毛囊生长、毛乳头细胞增殖和分泌血管内皮生长因子(VEGF)的影响。方法选择不同浓度黄芩苷作用于体外培养的人头皮毛囊8天,观察人毛囊生长及形态变化:作用于人毛乳头细胞72 h,四甲基偶氮唑监(MTF)法测定细胞增殖活性,ELISA法检测细胞分泌的VEGF水平。结果7.5.15,30,45μg/mL的黄芩苷对体外培养的人头皮毛囊有明显促生长作用;3.75,7.5,15,30μg/mL的黄芩苷对毛乳头细胞增殖无明显影响,但促进细胞分泌VEGF,与阴性对照组相比,差异有统计学意义。结论黄芩苷促进体外培养的人头皮毛囊生长,部分可能是通过增加毛乳头细胞分泌VEGF促使毛发生长。Objective To investigate the in vitro effects of baicalin on the growth of human hair follicles and secretion of vascular endothelial growth factor （VEGF） by human dermal papilla cells （ DPCs ）. Methods Human hair follicles at the anagen stage of hair cycle were obtained during operation from the occipitalia, cultured with baicalin of different concentrations （ 3.75, 7.5, 15, 30, 45, 60 μg/mL ）. Then, hair shait elongation and morphological changes in hair bulbs were observed and documented alter 1, 2, 4, 6 and 8 days of culture, DPCs were isolated from the human hair follicles, incubated with different concentrations （ 1, 3.75, %5, 15, 30, 45, 60, 80,100, 150 μg/mL ） of baicalin for 72 h. The rate of cell proliferation was measured by methyl thiazolyl tetrazolium （ MTT ）method. ELISA was used to detect the level of VEGF secreted by DPCs. Results Compared with the negative control, baicalin （ 7.5, 15, 30, 45 μg/mL ） significantly promoted the hair shait elongation of cultured human hair follicles （ all P 〈 0.05 ）. Baicalin at concentrations of 3.75, 7.5, 15 and 30 μg/mL had no significant effect on the proliferation of DPCs, but significantly elevated the secretion of VEGF by DPCs （ all P 〈 0.05 ）. Conclusion Baicalin can promote the growth of cultured human hair follicles, likely by stimulating the secretion of VEGF by DPCs.

关 键 词：毛囊 血管内皮生长因子类 黄芩甙 毛乳头细胞 

分 类 号：R285[医药卫生—中药学]