反相高效液相色谱法测定全血中环孢素的浓度  

Determination of Cyclosporin in Human Whole Blood by RP-HPLC

作　　者：李碧峰[1] 冯惠平[1] 贾晋蓉[1] 陈青青[1] 

机构地区：[1]漳州市医院药剂科,福建漳州363000

出　　处：《中国药师》2007年第7期649-651,共3页China Pharmacist

摘　　要：目的:建立一种简便可行的RP-HPLC法测定全血中环孢素(CsA)的浓度,为临床合理应用CsA提供参考。方法:全血样品1ml经乙醚单步提取,正已烷洗涤去干扰后采用C_(18)柱(200mm×4.6mm,5μm)分离;流动相为乙腈-甲醇-水(72:6:22);流速为1.5ml·min^(-1);柱温为60℃;检测波长为210nm;环孢素D(CsD)为内标。结果:CsA全血浓度在50～1000ng·ml^(-1)范围内呈良好的线性关系(r=0.9996),平均方法回收率为96.6%～99.2%,平均萃取回收率为59.5%～65.3%;日内、日间RSD均小于6%,最低检测限为10ng·ml^(-1)。结论:本法简便、快速、准确可靠,用于移植术后患者及再生障碍性贫血患者的CsA血药浓度监测,效果良好。Objective： To establish a practical RP-HPLC method to determine the whole blood concentration of cyclosporin A. Method： CsA was extracted from whole blood with diethyl and removed the endogenous interference in blood with n-hexane ether and separated. Diamonisi Cls column （ 200 mm × 4.6 mm,5 μm） was used ; mobile phase was acetonitrile- methanol-deionized water （72： 6： 22） ; the temperature of column was set at 60 ℃ ; The detecting wavelength was 210 nm; the internal standard was cyclosporin D. Result： A good linear relationship was obtained between the amount of CsA from 50 ng·ml^-1 to 1 000 ng.ml-1 （r =0.999 6）. The average method recovery rate of CsA was 96.6% - 99.2% , the average extraction recovery rate was 59.5% - 65,3% and the RSD with in-clay and between-clay were less than 6%. The detection limit of CsA was 10 ng·ml^ -1. Conclusion： The method is accurate,simple, sensitive and suitable for the monitoring blood-drug concentration of CsA after transplantation and aplastic anemia.

关 键 词：反相高效液相色谱法 环孢素 血药浓度监测 

分 类 号：R97[医药卫生—药品]