基于生物质谱技术的磺酸化修饰策略及其在蛋白质组学中的应用  

Sulfonation Strategy Based on Mass Spectrometry and Its Application in Proteomics Research

作　　者：赵丽艳[1] 周春喜[1] 张养军[1] 蔡耘[1] 钱小红[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《质谱学报》2007年第3期185-192,共8页Journal of Chinese Mass Spectrometry Society

基　　金：国重家点基础研究规划项目(2004CB520802);国家自然科学基金重点项目(20635010);国家自然科学基金(20405017;20505018;20505019);北京市科技计划重大项目(H030230280190)

摘　　要：对近几年报道的磺化修饰策略及其应用进行了评述。通过磺化修饰在肽段上引入带负电荷的磺酸基,不仅可以诱导肽段离子的质谱裂解,得到以y系列离子为主的序列信息丰富的串联质谱图,有利于肽段从头测序,还可以改变被修饰肽段的电荷分布,通过离子交换色谱法分离富集磺化修饰的肽段,有助于蛋白质鉴定。Negatively-charged sulfonic group introduced by sulfonation derivatization not only induced the MS/MS fragmentation of peptides and produced an exclusive series of y-type ion spectrum, but also changed the charge distribution of sulfonated peptides and made the enrichment of target peptides by ion-exchange chromatography possible. This technique enormously facilitates de novo peptide sequencing and protein identification by mass spectrometry. The procedure of sulfonation derivatization and its application in the study of proteomics are reviewed.

关 键 词：磺酸化 化学修饰 蛋白质组学 生物质谱 

分 类 号：Q503[生物学—生物化学] O657.63[理学—分析化学]