酶催化-荧光猝灭法测定药物中的万古霉素  

Determination of Vancomycin in Injection via Enzyme Catalysis-Fluorescence Quenching Method

作　　者：冯霞光[1] 张敏[1] 赵虎[1] 王怀友[1] 

机构地区：[1]山东师范大学化学化工与材料科学学院,济南250014

出　　处：《高等学校化学学报》2007年第7期1270-1272,共3页Chemical Journal of Chinese Universities

基　　金：山东省自然科学基金(批准号:Y2006B31)资助

摘　　要：A new method for determining vancomycin was established based on the principle of fluorescence quenching.The mechanism of the fluorescence quenching was discussed.A linear relationship is obtained between F0/F and the concentration of vancomycin in the range of 40.0—450.0 μg/mL with the correlation coefficiency 0.9991.The detection limit was 10.91 μg/mL and the relative standard derivative was 0.70%.The effects of pH,foreign ions,standing time on the determination of vancomycin were examined.The results obtained by this method were agreed with those by the official method.A new method for determining vancomycin was established based on the principle of fluorescence quenching. The mechanism of the fluorescence quenching was discussed. A linear relationship is obtained between Fo/F and the concentration of vancomycin in the range of 40. 0--450. 0μg/mL with the correlation coefficiency 0. 9991. The detection limit was 10. 91μg/mL and the relative standard derivative was 0. 70%. The effects of pH, foreign ions, standing time on the determination of vancomycin were examined. The results obtained by this method were agreed with those by the official method.

关 键 词：万古霉素 酪氨酸 辣根过氧化酶 H2O2 荧光猝灭 

分 类 号：O657.39[理学—分析化学]