成骨肿瘤细胞在纳米氧化钛陶瓷表面的生物活性研究  

Studies on the Bioactivity of Osteoblasts on the Surfaces of Nano-TiO_2 Ceramics

作　　者：屈阳[1] 李振声[1] 杨帮成[1] 张兴栋[1] 

机构地区：[1]四川大学生物材料工程研究中心,成都610064

出　　处：《高等学校化学学报》2007年第7期1288-1291,共4页Chemical Journal of Chinese Universities

基　　金：教育部留学回国人员基金项目(批准号:2004527-16-11);国家'八六三'计划项目(批准号:2004AA32G120);国家自然科学基金(批准号:50672062)资助

摘　　要：以羟基磷灰石和氧化镁为晶粒生长抑制剂制备的纳米氧化钛陶瓷为研究对象,采用体外成骨细胞Ros17/28与材料复合培养的方法,通过MTT法、荧光染色法和SEM细胞形貌观察等手段综合判断细胞在材料表面的活性,以此评价纳米氧化钛陶瓷的生物活性.结果表明,以羟基磷灰石为晶粒生长抑制剂的氧化钛陶瓷晶体颗粒尺寸达到纳米级,其生物活性超出了以氧化镁为晶粒生长抑制剂的氧化钛陶瓷和纯羟基磷灰石陶瓷,具有优异的生物相容性,是生物活性陶瓷.Traditional TiO2 is bioinert, which could just form mechanical connection with the surrounding bone-tissue without bioactivity. Because of the nano-effect of nano-matefials, the properties of nano-material is always better than the traditional ones, which made it logical to assume that nano-technology is a potential method to improve the bioactivity of ceramics. In this paper, the bioactivities of nano-TiO2 ceramics prepared via normal sintering method by adding hydroxyapatite （HA） or MgO as the crystal growth inhibitors were studied with cell culture method, in which Ros17/28 （osteoblast） was cultured on nano-ceramics in vitro. The bioactivity and biocompatibility of nano-TiO2 ceramics were evaluated by investigating the cell extrinsic shape, integrality of cell, cell activity and proliferation by SEM observation and cell toxicity tests including fluorescence crystal coloration observation and MTF, respectively. The result shows that nano-TiO2 ceramics with HA as the inhibitor had a good biocompatibility. Its bioactivity is better than pure HA and TiO2 ceramics with MgO as the crystal growth inhibitor.

关 键 词：纳米氧化钛陶瓷 成骨细胞 细胞培养 生物活性 

分 类 号：R318.08[医药卫生—生物医学工程] O641[医药卫生—基础医学]