川芎嗪对大鼠脑缺血再灌注Bcl-2、c-fos、Caspase-3的影响  

The Effect of Tetramethylpyrazine on Bcl-2、c-fos、Caspase-3 in Rats Cerebral Ischemia Reperfusion

作　　者：肖成云[1] 刘军涛[1] 

机构地区：[1]荆门职业技术学院医学院,湖北荆门448000

出　　处：《河南中医学院学报》2007年第4期28-29,共2页Journal of Henan University of Chinese Medicine

摘　　要：目的:探讨川芎嗪干预脑缺血再灌注损伤后对Bcl-2蛋白、c-fos蛋白、Caspase-3蛋白的表达影响及其保护大脑损伤的作用机制。方法:将SD大鼠30只,随机分为3组,即假手术组、模型组和治疗组,每组10只。建立脑缺血再灌注模型。治疗组用川芎嗪干预,采用免疫组化法测定川芎嗪干预大鼠脑缺血24h后对Bcl-2蛋白、c-fos蛋白、Caspase-3蛋白表达的变化。结果:在脑缺血再灌注损伤24h后,治疗组脑组织Bcl-2蛋白阳性神经元数较模型组明显增多,差异有显著性意义(P<0.01);治疗组c-fos蛋白和Caspase-3蛋白表达阳性神经元数较模型组明显减少,差异有显著性意义(P<0.01)。结论:川芎嗪能上调Bcl-2蛋白和下调c-fos蛋白和Caspase-3蛋白表达,川芎嗪可能通过抑制脑缺血再灌注损伤后细胞凋亡机制,从而对脑脑缺血再灌注损伤有保护作用。Objective： To explore the effect of tetramethylpyrazine on the expression of Bcl-2, c- los and Caspase- 3 proteins and protective cerebral mechanism after cerebral ischemia/reperfusion. Methods. Thirty SD rats were selected and randomized into three groups, sham group, model group and treatment group, each involved ten rats. The cerebral ischemia reperfusion models were established. The expression of Bcl - 2, e - fos and Caspase- 3 proteins were detected using immunohistochemical method 24 h after cerebral isehemia/reperfusion treated by tetramethylpyrazine in the treatment group. Results： The expression of Bcl - 2 proteins in the treatment group were significantly higher than those in the model group （P 〈 0. 01）. While the expression of e - Fos and Caspase - 3 proteins in the treatment group were significantly lower than thee in the model group （P 〈0.01）. Conclusion. tetramethylpyrazine can upregulated the expression of Bcl - 2 protein and downregulated the expression of e - Fos and Caspase- 3 proteins, tetramethylpyrazine may protect the cerebral effect on ischemia reperfusion injury through inhibiting neuron apoptosis.

关 键 词：川芎嗪 脑缺血再灌注 BCL-2 C-FOS CASPASE-3 凋亡 

分 类 号：R285[医药卫生—中药学]