一株具有抗稻瘟霉活性的海洋细菌32-11-2-2的筛选和分离鉴定  

Screening,identification and classification of an antimitosis marine bacterium from Eastern China Sea

作　　者：杨妤[1] 许强芝[1] 艾峰[1] 刘小宇[1] 施晓琼[1] 焦炳华[1] 

机构地区：[1]第二军医大学基础部生物化学与分子生物学教研室,上海200433

出　　处：《第二军医大学学报》2007年第7期718-721,共4页Academic Journal of Second Military Medical University

基　　金：上海市卫生系统百名跨世纪优秀学科带头人培养基金(05XD14023);国家高技术研究发展计划(863计划)(2006AA09Z416;2006AA09Z425).~~

摘　　要：目的:从中国东海微生物样品中分离筛选活性细菌菌株,并对活性菌株32-11-2-2进行鉴定。方法:采用无限稀释和平板划线法分离微生物样品,利用稻瘟霉模型进行活性筛选;并对其中的活性菌株32-11-2-2的形态、培养特征以及生理生化特征进行研究,使用16S rDNA序列分析的方法对菌株32-11-2-2进行了分类鉴定。结果:分离得到421株细菌,包括活性菌株54株,其中活性菌株32-11-2-2是一株弱嗜盐菌,具有陆地芽孢杆菌的形态特点,能产生胞外淀粉酶、过氧化氢酶,液化明胶、石蕊牛奶反应阳性,16S rDNA序列分析该菌株与枯草芽孢杆菌(Bacillus subtilis)同源性达到98%以上。结论:活性菌株32-11-2-2是一株适应了海洋环境的枯草芽孢杆菌。Objective： To screen,identify and classify an antimitosis marine bacterium strain 32-11-2-2 of Eastern China Sea. Methods： The marine bacteria were isolated with the infinite dilution and plate streak technique and screened for antimitotic active strains by Pyricularia Oryzae model. The active strain 32-11-2-2 was subjected to morphological, physiological and biochemical study, and was classified by the analysis of the 16S rDNA sequence. Results： A total of 421 strains of marine bacteria were isolated from marine water and sediment samples; 54 of them had antimitotic activity. The study of morphological, physiological and biochemical characteristics showed that the strain 32-11-2-2 was a slight halophilic strain; it had the morphological characteristics of a continent Bacillus and could produce amylase, fluidify glutin; and it also showed a positive reaction in the litmus milk test. The 16S rDNA analysis showed the strain 32-11-2-2 had a 98% homology with Bacillus subtilis. Conclusion： The strain 32-11-2-2 has been identified to be a Bacillus subtilis adapted to the oceanic environment.

关 键 词：海洋生物学 芽孢杆菌 枯草 活性筛选 鉴定 

分 类 号：R282.77[医药卫生—中药学]