猪流感病毒分离株HA基因的克隆与序列分析  

Cloning and sequence analysis of HA gene of swine influenza H3N2 subtype virus isolated from China

作　　者：廖晓萍[1] 刘文字[1] 周庆丰[2] 马静云[2] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]华南农业大学动物科学学院,广东广州510642

出　　处：《中国兽医科学》2007年第7期574-577,共4页Chinese Veterinary Science

基　　金：广东省科技计划项目(2004A2090102)

摘　　要：从1株H3N2亚型猪流感病毒(SIV)中提取RNA,用RT-PCR方法扩增了其HA基因的全长cDNA片段,克隆后测定其核苷酸序列,并推导了相应的氨基酸序列。结果,扩增的H3N2亚型SIV HA基因长度为1 701个核苷酸,共编码566个氨基酸。核苷酸序列与已发表的中国香港、中国大陆、美国及欧洲分离的H3亚型毒株相近,核苷酸和氨基酸序列相似性均在97%以上,同属H3亚型。其HA1、HA2之间切割位点序列为PSIQSR↓G,从分子水平推论,属于非高致病力毒株。其推导的氨基酸序列中有8个糖基化位点,7个位于HA1基因的第8、22、38、63、126、165、285位点,1个位于HA2基因的154位点。研究数据已提交至GenBank,并申请到一个登录号EF569597。研究结果为我国流感病毒基因库的建立、流感疫情的监测和防制提供了理论依据和技术资料储备。The HA gene of H3N2 subtype swine influenza virus（SIV） isolated from Guangdong Province was successfully amplified by RT-PCR. The amplicon was 1 701 bp in length,coding for 566 amino acid residues,and had 97% of identity with the H3 strains from China mainland, Hongkong, the United States and Europe. Sequence analysis revealed that cleavage site between HA1 and HA2 was PSIQSR↓G ,and the deduced peptide of 556 amino acid residues contained 8 glycosylation sites. Seven of them existed at the sites of 8,22,38,63,126,165 and 285 in the HA1 gene,and the other existed at the site of 154 in the HA2 gene. The research data had been submitted to GenBank and gained a new accession No. EF569597. The result provided the foundation for the establishment of our country＇s gene bank and monitoring-control of epidemic influenza viruses.

关 键 词：猪流感病毒 HA基因 克隆 序列分析 

分 类 号：S852.659.5[农业科学—基础兽医学] Q785[农业科学—兽医学]