波尔山羊卵母细胞和早期胚胎的端粒酶活性  

Detection of telomerase activities in the Boar goat oocyte and embryos during early development using TRAP assay

作　　者：敬晓棋[1] 雷安民[1] 屈雷[2] 窦忠英[1] 

机构地区：[1]西北农林科技大学动物科技学院国家干细胞工程研究中心陕西分中心,陕西杨凌712100 [2]榆林学院生命科学学院,陕西榆林719000

出　　处：《中国兽医科学》2007年第7期627-630,共4页Chinese Veterinary Science

基　　金：国家高技术研究发展计划(863)项目(2002AA216161);国家自然科学基金项目(302000137);国家教育部重大项目(03160)

摘　　要：利用端粒重复序列扩增(TRAP)法进行了山羊卵母细胞和附植前胚胎的端粒酶活性的测定;结果表明,山羊卵母细胞和早期胚胎的端粒酶活性都为阳性。TPG定量比较发现,卵母细胞的TPG最低,为25.348U;4-细胞的TPG为273.832U,至8-细胞时,TPG又降低到56.117U,随后快速升高,桑椹胚的TPG为251.111U;囊胚的端粒酶活性最高,TPG为519.46U。The telomerase activities in Boar goat oocyte and preimplantation embryos were detected by PCR based on the telomeric repeat amplification protocol （TRAP）. Results showed that the telomerase activities were positive in Boar goat oocyte and all early developmental stage embryos. The quantitative comparison carried out by the ladder densitomitric method showed that the total product generated（TPG） were 25. 348,273. 832,56. 117,251. 111 and 519.46U in Boar goat oocyte, 4-cell embryo, 8-cell embryo, morulae and blastocyst, respectively.

关 键 词：端粒酶活性 波尔山羊 早期胚胎 端粒重复序列扩增法 

分 类 号：S814.8[农业科学—畜牧学]