女性下生殖道炎症患者五种支原体的检测分析  

Analysis of the detected results for five different Mycoplasmas in female patients with lower genital tract infection

作　　者：曹淑彦[1] 周丽萍[1] 蔡晓红[1] 周铁丽[2] 

机构地区：[1]温州医学院第二附属医院科研中心,浙江温州325027 [2]温州医学院第一附属医院检验科,浙江温州325027

出　　处：《温州医学院学报》2007年第4期340-343,共4页Journal of Wenzhou Medical College

基　　金：浙江省自然科学基金资助项目(399066)。

摘　　要：目的:探讨女性下生殖道炎症患者发酵支原体(Mf)、穿通支原体(Mpe)、梨支原体(Mpi)、解脲脲原体(Uu)以及人型支原体(Mh)的感染情况,为临床诊治提供依据。方法:病例组(280例,其中宫颈炎120例,阴道炎100例,宫颈炎合并阴道炎60例)和对照组(280例)的宫颈或阴道分泌物,分别接种于改良SP-4和Uu、Mh培养基进行分离培养。培养阳性菌株采用生化反应试验、套式聚合酶链反应(nPCR)和核酸序列测定予以鉴定。结果:①病例组分离出阳性菌株144株(占51.43%),其中Mf8株(占2.86%),Mpe7株(占2.50%),Uu75株(占26.79%),Mh54株(占19.29%),未分离出Mpi;对照组仅分离出5株Uu(占1.79%)。系列鉴定结果与培养结果一致。②病例组与对照组在发酵支原体和穿通支原体的分离率上有统计学意义(P<0.01)。③宫颈炎组与阴道炎组间支原体的总分离率差异有显著性(P<0.01)。结论:女性下生殖道炎症与五种支原体感染有关;妇科炎症患者存在AIDS相关支原体感染,在临床诊治中应给予足够关注。Objective：To investigate the infectious states of Mf, Mpe, Mpi, Uu and Mh in female patients with lower genital infection and to provide some information for clinical diagnosis and therapy. Methods： The cervical or vaginal secretions were collected from 280 patients and 280 healthy controls, and the samples were inoculated into modified SP-4 culture medium, Uu and Mh media respectively for isolating. Meanwhile, biochemical tests, nested polymerase chain reaction （nPCR） and nucleotide sequence assay were performed to affirm the AIDS -associated mycoplasmas. Results： （1）Eight strains of Mf （2.86%）, 7 strains of Mpe （2.50%）, 75 strains of Uu （26.79%）, 54 strains of Mh （19.29） and 0 strains of Mpi （0%） were isolated from patients. But only 5 strains of Uu （1.79%） were isolated in healthy controls. The results of identify were sere as the isolation culture. （2）There was striking difference in statistics between the isolation rates of patients and healthy control; and （3）There was also difference for the total isolation rate between the cervicitis patients group and the vaginitis patients group. Conclusion： There are correlation between the female lower genital tract inflation and five different mycoplasmas infection. AIDS-associated mycoplasmas are found in patients with gyrecological inflammation, we should pay enough attention in clinical works.

关 键 词：发酵支原体 穿通支原体 梨支原体 解脲脲原体 人型支原体 宫颈炎 阴道炎 

分 类 号：R711.3[医药卫生—妇产科学] R375.3[医药卫生—临床医学]