ACEI对自发性高血压大鼠颈动脉血管平滑肌p53基因表达的干预作用  

The Effect of ACEI on Expression of p53 gene in Carotid Smooth Muscle from the Spontaneous Hypertension Rat

作　　者：向前 刘洪 孙明[3] 

机构地区：[1]深圳市光明医院,广东深圳518107 [2]深圳市西丽医院,广东深圳518051 [3]中南大学附属湘雅医院,湖南长沙410078

出　　处：《湖北民族学院学报（医学版）》2007年第2期32-34,共3页Journal of Hubei Minzu University(Medical Edition)

基　　金：深圳市科技局资助课题(980013)

摘　　要：目的探讨血管紧张素酶抑制剂(ACEI)对自发性高血压大鼠颈动脉中p53基因mRNA表达的影响。方法自发性高血压大鼠(SHR)实验前检测mRNA的表达情况,实验组随机分为蒙诺组(M组)、科素雅组(K组)、硝苯地平组(Ca2+组),分别加用蒙诺2mg/(kg.d-1),科素亚10mg/(kg.d-1),硝苯地平2mg/(kg.d-1)饲养12周。用逆转录聚合酶链式反应检测基因的表达水平。正常雄性大鼠(WKY)作为对照。结果SHR颈动脉中,p53基因有明显的高表达,较WKY有显著差异(P<0.05)。治疗12周后,p53基因在M组中表达明显减弱,较K组、Ca2+组有显著差异(P<0.05)。结论自发性高血压大鼠颈动脉组织中抑癌基因p 53可能与其颈动脉血管重构有关,ACEI可能通过对p53等抑癌基因表达的抑制作用逆转血管重构。Objective To study the effect of angiotensin enzyme inhibitor（ACEI） on the mRNA expression of p53 gene in carotid smooth muscle from the spontaneous hypertension rat （SHR）. Methods Check the mRNA expression before treatment, then SHR divide randomly into M （Monopril）, K（Cozaar） and Ca^＋ （Nifedipine） groups. SHR was treated with Monopril 2 mg/ （ kg · d^-1 ） ,Cozaar 10 mg/（ kg · d^-1 ） and Nifedipine 2 mg/（ kg · d^-1 ） for 12 weeks. The mRNA expression of p53 were examined by RT - PCR. As control group, WKY was experimented too. Result The mRNA of both of the two genes expressed obviously in SHR, and had evidently difference comparing to WKY＇（P 〈0.05） ; After 12 weeks treatment, the mRNA expression of p53 gene had been weakened obviously, and had evidently difference comparing to K and Ca^＋ groups＇ （P 〈 0. 05）. Conclusion The expression of tumor suppressor gene p53 probably relate to carotid reconstruction;Angiotensin enzyme inhibitor probably reverse the reconstruction by inhibiting the expression of p53 gene.

关 键 词：血管紧张素酶抑制剂 P53基因 血管重构 

分 类 号：R544.1[医药卫生—心血管疾病]