血清酶检测线性评价研究  

The Linear Evaluation of Serum Enzyme detection

作　　者：冯海螺 毕晓云 吴志东 

机构地区：[1]广州经济技术开发区医院,广东广州510730

出　　处：《湖北民族学院学报（医学版）》2007年第2期59-60,共2页Journal of Hubei Minzu University(Medical Edition)

基　　金：广东省社会发展领域科技计划项目(63104)

摘　　要：目的对临床酶学检测的线性范围进行评价和确认,保证线性范围内的检测结果准确可靠。方法利用临床新鲜血清标本配制成系列活性浓度样本,在Hitachi 7060生化仪上对各样本的丙氨酸氨基转移酶(ALT)进行检测,通过数据统计和处理,确认ALT的检测线性范围。结果ALT的线性范围可达1190 U/L。结论对定量检测项目均应确认其线性范围,避免线性以外的结果报告发出。Objective Evaluate and validate the linear scope of the clinical detection on enzyme, to ensure the detecting results be reliable in the linear scope. Method First, using the fresh blood serum from clinic to make serial samples for various activities. Secondly, make the alanine aminotransferase （ALT） detection on the samples in the Hitachi 7060 Biochemistry Meter. Then, statistical analysis the detecting data to validate the linear scope of the ALT detecting value. Result The linear scope of the ALT detecting value can be 1190 U/L. Conclusion It＇s important to validate the linear scope for all the detecting value in the quantificational detection, avoid sending out the report with the value out of the linear scope.

关 键 词：丙氨酸氨基转移酶 线性范围 新鲜血清 

分 类 号：R446.11[医药卫生—诊断学]