玉郎伞多糖对过氧化氢诱导大鼠原代肝细胞损伤的保护作用  

Protective Effects of Yulangsan Polysaccharide(YLS) on Primary Cultured Rat Hepatocytes Injury Induced by H_2O_2

作　　者：段小群[1] 焦杨[2] 张士军[2] 黄仁彬[2] 

机构地区：[1]桂林医学院,广西桂林541004 [2]广西医科大学,广西南宁530021

出　　处：《时珍国医国药》2007年第7期1592-1593,共2页Lishizhen Medicine and Materia Medica Research

基　　金：广西自然科学基金项目(No.9912038)

摘　　要：目的研究玉郎伞多糖（YLS）对H2O2诱导大鼠原代肝细胞损伤的保护作用及其机理。方法采用IV型胶原酶灌流法分离大鼠肝细胞进行原代培养,用H2O2体外诱导肝细胞损伤,检测培养上清液中AST和ALT水平,测定肝细胞中MDA和GSH含量,MTT法检测细胞存活和增殖活性。结果YLS（0.125～1 mg/ml）可明显降低或恢复由H2O2升高的培养上清液中AST和ALT水平及肝细胞中MDA含量,还可提高H2O2降低的肝细胞存活率和GSH含量。结论提示YLS对大鼠原代培养肝细胞损伤有直接保护作用,该作用可能与其抗氧化作用有关。Objective To study the protective effect and its mechanism of Yulangsan polysaccharide（YLS） on primary cultured rat hepatocytes injury induced by H2O2. Methods The primary rat hepatocytes were isolated by perfusion with IV collaganase and injured by H2O2. The contents of malondialdehyde（MDA） and glutathion（GSH） in hepatocytes and the levels of AST and ALT in cultural supernatant were determined by general methods. Cell viability was assayed by MTI＂ method. Results The elevation of MDA content in hepatocytes and AST and ALT levels in supernatant of cultural hepatocytes , and the decrease in cell viability and GSH content induced by H2O2 were restored remarkably by the treatment with YLS（0. 125 - 1 mg/ml）. Conclusion The results suggest that YLS possesses direct protective action on primary hepatocyte injury induced by H2O2. This might be associated with the anti - oxidative activity of YLS.

关 键 词：玉郎伞多糖 肝细胞 过氧化氢 抗氧化作用 

分 类 号：R285.5[医药卫生—中药学]