酸性染料比色法测定虎舌红中总生物碱的含量  

Determination of the Total Alkaloids in Ardisia mamillata by Acid Dye Colorimetry

作　　者：凌育赵[1] 杨妙贤[1] 

机构地区：[1]仲恺农业技术学院,广东广州510225

出　　处：《时珍国医国药》2007年第7期1597-1598,共2页Lishizhen Medicine and Materia Medica Research

基　　金：仲恺农业技术学院科研基金资助项目(No.G3052727)

摘　　要：目的建立测定虎舌红总生物碱的含量测定方法。方法采用溶剂萃取酸性染料比色法测定,以去氢飞廉碱（C16H22N4O2）为对照品,溴甲酚绿为酸性染料,用氯仿萃取,检测波长418 nm。结果去氢飞廉碱在0.72～14.02 mg/L范围内与吸光度呈良好线性关系,回归方程为：Y=19.254X-1.6247,r=0.999 5（n=5）,平均回收率为101.65%,RSD为0.75%（n=5）。结论所建立方法简便、准确、选择性强。Objective To establish a method for quantitative determination of the total alkaloids in the root of Ardisia mamillata. Methods The content was determined by solvent extraction and acid dye colorimetiy, with Acanthoine Ruscope -ine （C16H22 N4O2 ） as a control criterion and bromocresol green as the acid dye, and the wavelength was at 418 nm. Results Acanthoine Ruscope - ine showed a good linear relationship with absorbance when its concentration was within 0.72 - 14.02 mg/L. The regression equation established was Y = 19. 254X - 1. 624 7, r = 0. 999 5 （ n = 5 ）. The recovery of Acanthoine Ruscope - ine was 101.65% ,RSD was 0.75% （n =5 ）. Conclusion This method is easy, accurate and specific.

关 键 词：虎舌红总生物碱 酸性染料比色法 去氢飞廉碱 

分 类 号：R284.2[医药卫生—中药学]