高效液相色谱法测定复方银杏叶片中银杏总黄酮的含量  被引量:7

Determination of Flavonoids in Compound Ginkgo biloba Leaves Tablets by HPLC

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作  者:李小燕[1] 潘洪平[2] 

机构地区:[1]广西民族大学化学与生态工程学院,广西南宁530006 [2]广西壮族自治区人民医院,广西南宁530021

出  处:《时珍国医国药》2007年第7期1599-1600,共2页Lishizhen Medicine and Materia Medica Research

基  金:广西科学基金资助项目(No.0342003-1)

摘  要:目的建立复方银杏叶片中银杏总黄酮含量测定方法。方法采用高效液相色谱法,用Symmetry-C18色谱柱(250mm×4.6 mm,5μm),以甲醇-0.4%磷酸溶液(52∶48)为流动相,流速为1.0 ml.min-1,检测波长360 nm,柱温35℃。结果该法线性关系良好,平均加样回收率为98.35%,RSD为1.17%(n=5)。结论该法操作简便,分离效果好,结果准确,专属性强,可作为复方银杏叶片的质量控制方法。Objective To establish the determination method of flavonoids in compound Ginkgo biloba Leaves Tablets . Methods The analysis was performed on a Symmetry-C18 column(4.6 mm × 250 mm,5 μm) with methanol -0.4% phosphoric acid( 52:48 ) as mobile phase at a flow rate of 1.0 ml· min^ -1 , and at a column temperature of 35℃. The detection wavelength was 360nm. Results The linearity of this method was good. The average recovery of added samples was 98.35 % with RSD 1.17 %. ( n = 5 ) . Conclusion The method is convient with a good separating degree for the determination of flavonoids and can be used to evaluate the quality of compound Ginkgo biloba Leaves Tablets.

关 键 词:复方银杏叶片 黄酮 槲皮素 山柰素 异鼠李素 高效液相色谱法 

分 类 号:R284.1[医药卫生—中药学]

 

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