尖吻蝮蛇毒活性肽K组分的研究  

The Study of Peptide in Agkistrod on Acutus Snake Venom

作　　者：雷丹青[1] 周先丽[1] 李映新[1] 

机构地区：[1]广西医科大学蛇毒研究所,广西南宁530021

出　　处：《时珍国医国药》2007年第7期1608-1610,共3页Lishizhen Medicine and Materia Medica Research

基　　金：广西壮族自治区自然科学基金资助项目(No.0575062)

摘　　要：目的从尖吻蝮蛇毒中分离纯化一种小分子活性肽K组分,测定其理化性质及生物学活性。方法经Sephadex G-75凝胶过滤,超滤,DEAE-Sepharose CL-6B离子交换层析法分离纯化活性肽K,采用高效液相鉴定纯度,用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)测定其分子量,等电聚焦法测定等电点,用比浊法测定其抗血小板聚集活性,通过鸡胚绒毛尿囊膜(CAM)实验检测K组分对血管生成的影响。结果从尖吻蝮蛇毒中分离相对分子量约为7 862D,等电点为4.29的组分。该组分能抑制由二磷酸腺苷(ADP)、胶原(collagen)、凝血酶(thrombin)诱导的血小板聚集并成剂量依赖性;具有抑制鸡胚尿囊膜血管生成的作用。结论从尖吻蝮蛇毒中纯化出活性肽K,该组分有很强的抗血小板聚集和抗血管生成作用。Objective To purify a peptide from Agkistrodon Acutus snake venom and analyse its characteristics and biological activities. Methods To extract snake venom through 75 gel filtration, uhrafihration and DEAE -Sepharose CL-6B ionexchange. The purified product was identified by HPLC. The molecular weight was determined by SDS-polyacrylamid gel electrphoresis. The isoelectric point was estimated by the isoelectric focusing electrophoresis. Platelet aggragation was measured by nephelometry. The antiangiogenesis effect in vivo of peptide on CAM assay was observed. Results The molecular weight of peptide purified from Agkistrodon Acutus snake venom was 7 862D. Its isoelectric point was pH4.29. This peptide could dose-dependently inhibited the platelet aggregation induced by ADP, collagen and thrombin. It inhibit the spontaneous angiogenesis of CAM. Conclusion The method has been proved to be successful for the purification of peptide that inhibits platelet aggregation and angiogenesis.

关 键 词：尖吻蝮蛇毒 血小板聚集抑制 抗血管生成 

分 类 号：R962[医药卫生—药理学]