高效液相色谱法测定银黄颗粒中绿原酸的含量  

Determination of Chlorogenic Acid in Yinhuang Particle by HPLC

作　　者：魏清[1] 康红英 

机构地区：[1]三峡大学第一临床医学院.湖北省宜昌市中心医院,湖北宜昌443003 [2]湖北省宜昌市药品检验所,443000

出　　处：《时珍国医国药》2007年第7期1689-1690,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的建立银黄颗粒中绿原酸的含量测定方法。方法HPLC法测定银黄颗粒中绿原酸的含量。采用C18柱,以乙腈-0.4%磷酸（10∶90）为流动相,检测波长为327nm。结果在0.164～0.984μg.ml-1范围内线性关系良好（r=1,n=6）,平均回收率为100.0%,RSD=0.8%（n=4）。结论该方法简便、准确,可为标准的修订提供可行的依据。Objective To establish the determination method for chlorogenic acid in Yinhuang Particle. Methods Determination of Chlorogenic Acid in Yinhuang Particle by HPLC with C18 column and acetonitrile-0.4% phosphoric acid（ 10： 90）as the mobile phase. The detection wavelengh was 327nm. Results The linear range was 0. 164 -0. 984μg·m^ -1 （r = 1 ,n =6）. The average recovery was 100.0% ,RSD = 0.8% （n = 4 ）. ConclusionThe method is simple and accurate. It can offer the feasible basis for revision of the standard.

关 键 词：高效液相色谱法 银黄颗粒 绿原酸 

分 类 号：R284.2[医药卫生—中药学]