高效液相法测定驻车丸中小檗碱的含量  

Determination of Ferulic Acid in Zhuche Pills by HPLC

作　　者：姚丹[1] 才玉光[1] 谢成国[1] 

机构地区：[1]锦州医学院,辽宁锦州121001

出　　处：《时珍国医国药》2007年第7期1692-1693,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的采用高效液相色谱法测定驻车丸中小檗碱的含量。方法色谱柱为Kromasil C18;以乙腈-0.05 mmol/ml磷酸二氢钾（28∶72）为流动相;检测波长为345 nm,外标法定量。结果小檗碱在0.3～0.75μg之间呈良好的线性关系,r=0.9993,平均回收率为99.42%,RSD=1.9%（n=7）。结论方法准确、稳定性好,可作为该复方制剂的质量控制方法。Objcetive An HPLC method for the determination of berberine hydroehloride in Zhuehe Pills was established. Methods A Kromasil C18 column was used with aeetonitrile-0.05mmol/L CH3 CN （28： 72） . Content was detected at a wavelength of 345nm. Results The linear range was 0.3 ～ 0.75μg with correlation coefficient, r = 0. 999 3. The average recovery rate was 99.42%, RSD = 1.9% （ n = 7）. The method was accurate and repeatable . Conclusion It can be used for quality control of Zhuehe Pills.

关 键 词：驻车丸 小檗碱 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]