一个属于Aux/IAA基因家族的毛白杨PtIAA1的克隆和序列分析(英文)  

Cloning and Sequencing of PtIAA1 from Populus tomentosa, a Member of the Aux/IAA Gene Family

作　　者：董秀春[1] 樊金会[1] 于学宁[1] 束怀瑞[2] 

机构地区：[1]山东农业大学林学院,泰安271018 [2]山东农业大学园艺工程学院,泰安271018

出　　处：《分子植物育种》2007年第4期565-571,共7页Molecular Plant Breeding

基　　金：This research was supported by postdoctoral foundation of Shandong Agricultural University.

摘　　要：基于NCBI数据库中的序列信息,我们利用PCR技术克隆到一个毛白杨(Populustomentosa)PtI-AA1基因。序列分析表明PtIAA1cDNA长度为946个碱基,编码一个由258个氨基酸组成的蛋白,该蛋白与PtIAA1、NtIAA28和AtIAA16蛋白的同源性分别高达97%、75%和74%。系统进化分析表明PtIAA1基因可能属于Aux/IAA基因家族中组的成员。与大多数的Aux/IAA基因家族中的基因一样,PtIAA1蛋白是一个短命核蛋白,拥有4个高度保守的区域。The PtIAA1 gene was amplified from Populus tomentosa based on the sequence information from NCBI database. Sequence analysis indicated that the PtIAA1 cDNA has 964 bp length and encodes a 258-amino acid protein that has 97%, 75% and 74% identity to PtIAA1, NtIAA28 and AtIAA16 respectively. Phylogenic analysis suggests PtIAA1 gene could belong to the group of the A ux/1AA gene family. As same as most of the members of the A ux/1AA gene family, PtIAA1 protein would be a short-lived nuclear protein and also would contain four highly conserved domains.

关 键 词：毛白杨(Populus tomentosa) PtIAA1 Aux/IAA基因家族 克隆和序列分析 

分 类 号：S792.11[农业科学—林木遗传育种]