草与日本看麦娘愈伤组织对绿黄隆的诱导抗性  

Inductive Resistance to Chlorsulfuron in Beckmannia syzigachne and Alopecurus japonicum in Vitro

作　　者：李宜慰[1,2] 陈以峰[1,2] 李永丰[1,2] 汤日圣[1,2] 梅传生[1,2] 

机构地区：[1]江苏省农业科学院农业现代化研究所 [2]江苏省农业科学院农业生物遗传生理研究所

出　　处：《江苏农业学报》1997年第2期85-89,共5页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金

摘　　要：通过继代草、日本看麦娘幼穗愈伤组织观察杂草对绿黄隆的诱导抗性特点。结果表明：继代过程中随着绿黄隆浓度逐渐增加，杂草愈伤组织内乙酰乳酸合酶（ＡＬＳ）对绿黄隆的敏感性（以ＩＣ５０值表示）明显下降，草愈伤组织经６～１２次继代，ＩＣ５０约从１μｇ／Ｌ增至３００μｇ／Ｌ；日本看麦娘愈伤组织经４～１０次继代，ＩＣ５０约从１μｇ／Ｌ增至１００μｇ／Ｌ以上。说明杂草愈伤组织对绿黄隆的诱导抗性明显增强，并且草的这种诱导抗性也在再生小植株上表达，表现为２００μｇ／Ｌ绿黄隆对抗性再生小植株的生长抑制减弱；１０００μｇ／Ｌ绿黄隆对抗性再生小植株的致死率明显变小。而在继代过程中绿黄隆并不显著改变这两种愈伤组织的生长状况。Characteristics of resistance response to chlorsulfuron was observed in young panicle calli of both weeds via subculture. During subculture sensitivity of acetolactate synthase (ALS) in both calli greatly decreased along with increase of chlorsulfuron concentration. IC 50 value (concentration causing 50% inhibition) of ALS activity increased from 1 0 μg/L to 300 μg/L after 6-12 subcultures in Beckmannia syzigachne , or from 1 0 μg/L to over 100 μg/L after 4-10 subcultures in Alopecurus japonicum , indicating that obvious development of the herbicide resistance has appeared in both calli. Moreover, this inductive resistance also in regenerated plantlets of Beckmannia syzigachne . Inhibition of resistant plantlet growth at 200 μg/L of chlorsulfuron lowered, and lethal rate of these plantlets at 1 000 μg/L of chlorsulfuron became smaller. Neither growth of both callus nor calli differentiation potential and plantlet development of Beckmannia syzigachne was influenced by chlorsulfuron during subculture.

关 键 词：Wang草 日本看麦娘 绿黄隆 抗性 组织培养 杂草 

分 类 号：S451.221[农业科学—植物保护] S482.4