反义核酸技术应用难点及研究进展  

The Clinical Problem and Research Progress of Antisensenucleic Acids Technique

作　　者：易浔飞[1] 兰小鹏[1] 

机构地区：[1]福建医科大学福总临床医学院全军医学检验研究所,福建福州350025

出　　处：《生物技术通讯》2007年第4期655-659,共5页Letters in Biotechnology

基　　金：福建省科技厅国际合作重点项目(2004I012)

摘　　要：反义核酸技术已被广泛用于治疗药物、药物靶点确认、探知病理基因的表达。目前对其作用原理的研究集中于其被吸收入细胞的机制、在细胞内的分布、反义核酸序列的最佳长度和性质,并针对体内可能抑制反义核酸活性的影响因素,采取了各种相应的反义核酸优化技术,如对反义核酸的化学修饰、联结高效的转运载体、确定最佳的反义结合位点等,通过这些技术来提高其体内稳定性、跨细胞转运的效率,识别靶序列的特异性,以获得更多更好的反义药物投入实用。Antisensenucleic acids technique has been widely used in developing new gene-therapy drug, to indentify medicine target, and to study patho-gene expressing. Accordong to some reaserch focus on how the antisensenucleic acids inhibit expression of specific genes that including cellular delivery, intracellular distribution, and select the optimal oligodeoxynucleotide（ODN） size. The development of antisense strategies has generated to overcome the barriers that may affect antisensenucleic acids reaction. These developments including chemical mordification of antisense oligodeoxynu- cleotides, more effective carrier-mediated delivery of oligonucleotides, and selecting accessible sites in the target gene. The use of upgrade ODN can improve stability in vivo, more effective cellular delivery, the specificity of binding target gene so that acauire more and more antisensenucleic acids to throw into utility.

关 键 词：反义核酸 反义寡脱氧核苷酸 RNA干扰 核酶 反义结合位点 

分 类 号：Q75[生物学—分子生物学]