皱纹盘鲍蛋白酶的研究  

THE STUDY OF PROTEASE IN HALIOTIS DISCUS HANNAI INO

作　　者：杨蕙萍[1] 童圣英[1] 王子臣 

机构地区：[1]大连水产学院,青岛海洋大学水产学院116023

出　　处：《水产学报》1997年第2期129-133,共5页Journal of Fisheries of China

摘　　要：利用分光光度计比色法测定了2－3cm的皱纹盘鲍（HaliotisdiscushannaiIno）的蛋白消化酶的最适温度、最适pH以及11种金属离子对其的影响。结果表明：在最适温度50℃下的活化能为4．89×10~4J／mol；最适pH为2．60和5．00。对其最适pH值的分析表明：皱纹盘鲍存在有胃蛋白酶，且活性颇高。在11种离子中，Cu^(2+)、Hg^(2+)、Ag^+对蛋白酶活性具有显著的抑制作用，而Fe^(3+)。Mg^(2+)则具有一定的促进作用。The potease of the Pacific abalone(Haliotis discus hannai Ino) was measured during the length of 2 ~ 3cm by means of spectrophotometer- The optimum temperature is 50℃, The optimum pH value is 2.60 and 5.00. Under the optional temperature, the active energy of protease was 4.89 ×10~4J/mol. Pepsin was detected and found that its activity was very high. The effects of eleven species metal ions were analysed, the result indiated that CU+ 'Hgl+ \ Ag+ were idsbital the aCtivity of protease and Ca2 Fe3 + promoted the activity.

关 键 词：皱纹盘鲍 蛋白酶 消化生理 

分 类 号：S968.325[农业科学—水产养殖] Q482[农业科学—水产科学]