铁配合物对胰岛素硝化的影响  

The effects of iron complexes on the nitration of insulin

作　　者：汪铁林[1] 池泉[2] 黄开勋[2] 

机构地区：[1]武汉工程大学化工与制药学院,湖北武汉430074 [2]华中科技大学化学系,湖北武汉430074

出　　处：《武汉工程大学学报》2007年第4期5-7,10,共4页Journal of Wuhan Institute of Technology

摘　　要：生物体内一氧化氮和超氧阴离子快速反应生成的过氧亚硝酸根离子是一种强细胞毒性物质,它能够硝化胰岛素酪氨酸残基.采用UV-VIS在体外研究了几种铁配合物对过氧亚硝酸根离子硝化胰岛素反应的影响,并对影响铁配合物催化活性的因素进行了讨论.在生理pH条件下,Fe3+DTPA和柠檬酸铁对硝化反应无明显影响,Fe3+EDTA可显著催化硝化反应.Peroxynitrite anion （ONOO-）, which is formed in biological systems by the rapid combination of nitric oxide （NO） and superoxide anion （O^2＋-）,is a potent and versatile endogenous reactant that can lead to the tyrosine residues of insulin nitration. The effects of several iron complexes on the nitration of insulin by peroxynitrite anion were investigated with UV -Vis in vitro, and the factors of the effect on the catalytic activity of iron complexes were discussed. The present results show that Fe^3＋ DTPA and ferric citrate have no obvious effect on the reaction of insulin with peroxynitrite anion, but Fe^3＋ EDTA can catalyze the reaction significantly at physiological pH value.

关 键 词：过氧亚硝酸根 胰岛素 硝化 铁配合物 

分 类 号：O611.3[理学—无机化学]